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MABT1550-25UL Anti-Lactadherin/MFGE8 Antibody, clone 5C5

Anti-Lactadherin/MFGE8 , clone 5C5, Cat. No. MABT1550, is a mouse monoclonal antibody that detects Lactadherin and is tested for use in Immunofluorescence and Western Blotting.

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MABT1550-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1550-25UL
      DescriptionAnti-Lactadherin/MFGE8 Antibody, clone 5C5
      Alternate Names
      • Lactadherin
      • MFGM
      • Milk fat globule-EGF factor 8
      • MFG-E8
      • SED1
      • Sperm surface protein SP47
      • PP47
      Background InformationLactadherin (UniProt: P79385; also known as MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Sperm surface protein SP47, PP47) is encoded by the MFGE8 gene (Gene ID: 397545) in Boar. MFG-E8 is a peripheral membrane glycoprotein that is widely expressed in many cell types. At its N-terminal region, it contains two EGF-like repeats (aa 2-41 and 44-88). The second EGF repeat contains an RGF integrin-binding domain that allows its interaction with and activation of Vβ3 and Vβ5 integrins. It also has two C-terminal F5/8 type C domains. The second F5/8 type C domain exhibits high affinity for binding to phosphatidylserine present in cell membranes. MFG-E8 displays high affinity for glycoproteins on egg surface and contributes to the ability of sperm to adhere to the egg in an integrin-independent manner. It has been reported that although MFG-E8 is not absolutely essential for fertilization, its absence is shown to reduce fertility in mice. It has also been implicated in a variety of other physiological functions. MFG-E8 can bind apoptotic cells via its F5/8C domains, and its RGD motif is shown to be essential for cell engulfment by macrophages. In the mammary glands, it is required for clearance of apoptotic cells during mammary gland involution at the end of lactation. Defects in or lack of MFG-E8 can cause the accumulation of apoptotic cell remnants, duct distension and mastitis. (Ref.: Zigo, M., et al. (2015). Cell Tissue Res. 359(3); 895-908; Ensslin, MA., and Shur, BD. (2007). Proc. Natl. Acad. Sci. USA 104(8); 2715-2720).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Lactadherin/MFGE8 , clone 5C5, Cat. No. MABT1550, is a mouse monoclonal antibody that detects Lactadherin and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Lactadherin/MFGE8 in HeLa cell lysate.

      Western Blotting Analysis: A representative lot detected Lactadherin/MFGE8 in Western Blotting applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

      Immunofluorescence Analysis: A representative lot detected Lactadherin/MFGE8 in Immunofluorescence applications (Zigo, M., et al. (2015). Cell Tissue Res. 359(3):895-908).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified Sperm surface protein from Boar.
      EpitopeUnknown
      Clone5C5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5C5 is a mouse monoclonal antibody that detects Sperm surface protein (Lactadherin).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Porcine
      Species Reactivity NoteHuman, Boar.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MFGE8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~47 kDa observed; 45.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDA-MB-231 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Lactadherin/MFGE8 in MDA-MB-231 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1550-25UL 04065267327822