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MAB3749 Anti-LEF-1 Antibody, transactivation domain, clone 3A12

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MAB3749
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3749
      Replaces04-1130
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-LEF-1 Antibody, transactivation domain, clone 3A12
      Alternate Names
      • Lymphoid Enhancer Factor 1
      Background InformationLymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), andorthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-LEF-1 Antibody, transactivation domain, clone 3A12 is validated for use in WB for the detection of LEF-1.
      Key Applications
      • Western Blotting
      Application NotesWestern blot

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells.
      Epitopetransactivation domain
      Clone3A12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
      Gene Symbol
      • LEF1
      • LEF-1
      • TCF1-alpha
      • TCF1ALPHA
      • DKFZp586H0919
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
      SIZE: 399 amino acids; 44201 Da
      SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
      SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
      TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
      SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3749 04053252398223

      Documentation

      Anti-LEF-1 Antibody, transactivation domain, clone 3A12 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-LEF-1 Antibody, transactivation domain, clone 3A12 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI- LEF1 (TRANSACTIVATION DOMAIN) - 4071268 4071268
      MOUSE ANTI- LEF1 (TRANSACTIVATION DOMAIN) -2696724 2696724

      References

      Reference overviewPub Med ID
      LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic.
      Riese, J, et al.
      Cell, 88: 777-87 (1997)  1997

      Show Abstract
      9118221 9118221
      LEF-1 contains an activation domain that stimulates transcription only in a specific context of factor-binding sites.
      Giese, K and Grosschedl, R
      EMBO J., 12: 4667-76 (1993)  1993

      Show Abstract
      8223476 8223476

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies