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MABS2231-100UG Anti-Intestinal Stem Cell Antibody, clone F5C12

Anti-Intestinal Stem Cell, clone F5C12, Cat. No. MABS2231, is a rat monoclonal antibody that detects Intestinal stem cells and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).

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MABS2231-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2231-100UG
      DescriptionAnti-Intestinal Stem Cell Antibody, clone F5C12
      Alternate Names
      • Lgr+ Intestinal stem cells
      Background InformationThe small intestine consists of continuous villi and crypts. The crypt is mainly a proliferative compartment and is maintained by multipotent stem cells whereas the villus represents the differentiated compartment, and it receives cells from multiple crypts. The functional compartment of the epithelium contains differentiated cells that populate the villi and can be categorized based on their function: enterocytes that function to absorb nutrients, goblet cells that secrete a protective mucus barrier, and enteroendocrine cells that release gastrointestinal hormones. Intestinal crypts contain intestinal stem cells (ISC), which are multipotent adult stem cells that reside in the base of crypts. They continuously self-renew by dividing and differentiate into the specialized cells of the intestinal epithelium, which renews throughout life. ISCs located either at the crypt base interspersed between the Paneth cells can be categorized into active-cycling (Lgr5+ cells) or those located near the þ4 position in the crypt as slow-cycling Bmi-1+ cells. Paneth cells that are responsible for secreting antibacterial peptides reside at the base of the proliferative compartment. As ISCs begin to differentiate, they migrate toward the lumen and are eventually shed, either from the tip of the intestinal villi or from the surface of the colonic epithelium. Clone F5C12 is suitable for detecting Lgr5+ cells and facilitates enrichment of Lg5GFP cells by FACS analysis. It does not detect Bmi-1+ or Lyz1 marker for Peneth cells. (Ref.: Smith, NR., et al. (2018). Cell. Mol. Gastroenterol. Hepatol. 6(1);79-96; Umar, S. (2010). Curr. Gastroenterol. Rep. 12(5); 340-348).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Intestinal Stem Cell, clone F5C12, Cat. No. MABS2231, is a rat monoclonal antibody that detects Intestinal stem cells and is tested for use in Flow Cytometry and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Intestinal Stem Cell in Flow Cytometry applications (Smith, N.R., et al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

      Immunohistochemistry Applications: A representative lot detected Intestinal Stem Cell in Immunohistochemistry applications (Smith, N.R., et al. (2018). Cell Mol Gastroenterol Hepatol. 6(1):79-96).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenUndifferentiated Mouse intestinal epithelial cells. Rats were preimmunized with isolations of differentiated mouse intestinal epithelial cells and then treated with cyclophosphamide (i.p.) to eliminate B lymphocytes reacting against these antigens. Subsequent immunization was with crypt-based single cells.
      EpitopeUnknown
      CloneF5C12
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone F5C12 is a rat monoclonal antibody that detects Intestinal stem cells.
      IsotypeIgG2c
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse intestinal tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected Intestinal stem cell in mouse intestinal tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2231-100UG 04065267780719