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MAB2252Z-I Anti-Integrin β1 Antibody, clone N29 (Azide Free)

This Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: LAMB2, S-laminin, LAMS UniProt Number: P55268
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MAB2252Z-I
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, IP, FUNC, CULT, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2252Z-I
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone N29 (Azide Free)
      Alternate Names
      • CD29
      • MAB2252
      References
      Product Information
      FormatPurified
      Control
      • Tonsil, human skin, human kidney tissue
      • U251 cell lysate
      PresentationPurified cultured supernatant in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      • Cell Culture
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

      Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

      Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

      Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.

      Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenJurkat T-leukemic cell line
      CloneN29
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
      IsotypeIgGκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMB2
      • S-laminin
      • LAMS
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55268 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 1798 amino acids; 195981 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-2 is a subunit of laminin-3 (S-laminin), laminin-4 (S- merosin), and laminin-7 (KS-laminin).
      SUBCELLULAR LOCATION: Secreted, extracellular space. Note=S- laminin is concentrated in the synaptic cleft of the neuromuscular junction.
      DOMAIN: SwissProt: P55268 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
      DISEASE: SwissProt: P55268 # Defects in LAMB2 are the cause of Pierson syndrome [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. & Defects in LAMB2 are a cause of congenital nephrotic syndrome [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
      SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
      Molecular Weight88 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C in undiluted aliquots.
      Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2252Z-I 04053252934605

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies