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MAC139 Anti-Inntag™ 6 (IT6) Antibody, clone R19/4-11/15

Anti-Inntag 6 (IT6), clone R19/4-11/15 is a mouse monoclonal antibody that detects the Innocuous Inntag IT6 wherein the tag protein is a fragment of Hev b 6.02 of Hevea brasiliensis. It has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: HEV1 UniProt Number: P02877
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MAC139
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAC139
      DescriptionAnti-Inntag™ 6 (IT6) Antibody, clone R19/4-11/15
      Alternate Names
      • Major hevein
      Background InformationPro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag IT6 is based on a packed beta-sheet conformation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Inntag 6 (IT6), clone R19/4-11/15 is a mouse monoclonal antibody that detects the Innocuous Inntag IT6 wherein the tag protein is a fragment of Hev b 6.02 of Hevea brasiliensis. It has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected Inntag 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

      Immunoprecipitation Analysis: A representative lot detected Inntag 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

      Immunofluorescence Analysis: A representative lot detected Inntag 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
      Biological Information
      ImmunogenHis-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis.
      CloneR19/4-11/15
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6.
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Species Reactivity NoteHevea brasiliensis.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HEV1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~34.1 kDa observed; 21.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 6 (IT6) in 10 µg lysate from HEK293 cells transfected with GFP-IT6.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC139 04054839240577