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ABF188-25UG Anti-IRF-1

Anti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-IRF-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IRF1 UniProt Number: P10914
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ABF188-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF188-25UG
      DescriptionAnti-IRF-1
      Alternate Names
      • Interferon regulatory factor 1
      • IRF1
      Background InformationInterferon regulatory factor 1 (UniProt: P10914; also known as IRF-1) is encoded by the IRF1 gene (Gene ID: 3659) in human. IRF-1 is a transcriptional regulator that is expressed constitutively in all cells types except early embryonal cells. It can exist as a monomer and as a homodimer. It displays remarkable functional diversity in the regulation of cellular responses, including the regulation of interferon (IFN) and IFN-inducible genes, host response to viral and bacterial infections. It also regulates many genes expressed during hematopoiesis, inflammation, immune responses, cell proliferation and differentiation, and growth arrest and apoptotic cell death following DNA damage. IRF-1 is reported to stimulate p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Its DNA-binging domains are located in the first 115 amino acids of N-terminal region and contains a series of five well-conserved tryptophan-rich repeats. IRF-1 can be phosphorylated by casein kinase 2, which positively regulates its activity. Mutations in IRF1 gene are reported to cause gastric cancer. (Ref.: Dou, L., et al. (2014). Hum. Immunol. 75(11); 1110-1114).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected IRF-1 in Jurkat cell lysate.

      Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IRF-1 in human stomach cancer and human small intestine tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Interferon Regulatory Factor 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Interferon Regulaotry Factor 1 in human cells. It targets an epitope within 14 amino acids from the N-terminal half.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat, Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRF1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~48 kDa observed; 36.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-1 in PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF188-25UG 04054839448089