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ABS1623 Anti-HRD1 Antibody

Anti-HRD1 Antibody is an antibody against HRD1 for use in Western Blotting.

Anti-HRD1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Apoptosis - Additional Gene Symbol: SYVN1, HRD1, KIAA1810 UniProt Number: Q86TM6
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ABS1623
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1623
      DescriptionAnti-HRD1 Antibody
      Alternate Names
      • E3 ubiquitin-protein ligase synoviolin
      • Synovial apoptosis inhibitor 1
      Background InformationE3 ubiquitin-protein ligase synoviolin (UniProt Q86TM6; also known as Synovial apoptosis inhibitor 1) is encoded by the SYVN1 (also known as HRD1, KIAA1810) gene (Gene ID 84447) in human. E3 ubiquitin-protein ligase synoviolin is a multi-transmembrane ER membrane protein. Originally named Hrd1, synoviolin adopted the current name due to its upregulation in synovial fibroblasts from patients with rheumatoid arthritis as a result of exposure to inflammatory cytokines. Synoviolin plays a protective role against ER stress-induced apoptosis by mediating not only the degradation of ER misfolded proteins, but also the degradation of proapoptotic protein p53 as well as IRE1α (inositol-requiring enzyme 1α), a critical kinase regulating ER stress-induced cell death. Synoviolin is produced with a signal peptide (a.a. 1-22) sequence, the removal of which yields the mature 5-transmembrane (a.a. 42-62, 99-119, 141-161, 170-190, 225-245) protein, having its N-terminal end in the ER lumen (a.a. 23-41) and C-terminal end (a.a. 246-617) in the cytoplasm. Its C-terminal cytoplasmic end contains the p53/TP53-interacting region (a.a. 236-270), an atypical RING-type zinc finger domain (a.a. 291-330), and a proline-rich region (a.a. 339-478).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 155 mM NaCl, 50 mM Tris-HCl pH 7.4 with 0.05% sodium azide and 10% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HRD1 Antibody is an antibody against HRD1 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:200 dilution from a representative lot detected HRD1 in 10 µg of αT3-1 mouse pituitary gonadotroph cell lysate (Courtesy of Dr. Richard Wojcikiewicz, SUNY Upstate Medical University, Syracuse, NY).
      Western Blotting Analysis: A representative lot detected HRD1 in the membrane, but not the cytosolic, fraction from RAT-1 rat fibroblasts (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
      Western Blotting Analysis: A representative lot detected HRD1 in RAT-1 and HeLa whole cell lysates (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
      Western Blotting Analysis: A representative lot detected HRD1 in SPFH2 immunoprecipitate from αT3-1 mouse pituitary gonadotroph cell lysate. The amount of co-immunoprecipitated HRD1 increased when using lysates from cells treated with proteasome inhibitor bortezomib (Cat. No. 504314) to increase cellular accumulation of ubiquitinated SPFH2 (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminal sequence of human HRD1.
      EpitopeC-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityImmunogen sequence is present in all three spliced isoforms of human HRD1/SYVN1 reported by UniProt (Q86TM6).
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYVN1
      • HRD1
      • KIAA1810
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~74 kDa observed. 65.48 kDa (isoform 1), 59.53 kDa (isoform 2), and 65.41 kDa (isoform 3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in αT3-1 mouse pituitary gonadotroph cell lysate.

      Western Blotting Analysis: A 1:200 dilution of this antibody detected HRD1 in 10 µg of αT3-1 mouse pituitary gonadotroph cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1623 04055977344080