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MABE968 Anti-HLCS Antibody, clone 2C2.1

Anti-HLCS Antibody, clone 2C2.1 is an antibody against HLCS for use in Western Blotting.

Anti-HLCS Antibody, clone 2C2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: HLCS UniProt Number: P50747
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MABE968
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE968
      DescriptionAnti-HLCS Antibody, clone 2C2.1
      Alternate Names
      • Biotin--protein ligase
      • HLCS
      • Biotin apo-protein ligase
      • Biotin--[methylmalonyl-CoA-carboxytransferase] ligase
      • Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase
      • Holocarboxylase synthetase
      • HCS
      • Biotin--[methylcrotonoyl-CoA-carboxylase] ligase
      • Biotin--[acetyl-CoA-carboxylase] ligase
      Background InformationBiotin-protein ligase (EC 6.3.4.9; EC 6.3.4.10; EC 6.3.4.11; EC 6.3.4.15; UniProt P50747; also known as Biotin-[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase, Biotin-[methylcrotonoyl-CoA-carboxylase] ligase, Biotin-[methylmalonyl-CoA-carboxytransferase] ligase, Biotin-[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, HCS, Holocarboxylase synthetase) is encoded by the HLCS (also known as HCS) gene (Gene ID 3141) in human. HLCS (holocarboxylase synthetase) is the sole biotin protein ligase encoded by the human genome. HLCS catalyses the covalent modification of target lysine residues by biotin. In addiition to taregting various carboxylases in the cytoplasm and mitochondria, both the full-length HLCS and a splicing variant are reported to enter the nucleus and associate with multi-protein gene repression complex. HLCS overexpression in HEK293 cells is reported to downregulate histone H3 lysine9 acetylation (H3K9ac) level with a cocomitant drop in LTRs (long terminal repeats) 15 and 22 due to a decrease in mRNA coding for LTRs. HLCS is also reported to induce RANTES expression in HEK-293 cells by biotinylating heat shock protein 72 (HSP72).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HLCS Antibody, clone 2C2.1 is an antibody against HLCS for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminal region of human HLCS.
      EpitopeN-terminal region
      Clone2C2.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HLCS
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected HLCS in 10 µg of human skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE968 04055977300093