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119101 Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb

Anti-H+,K+-ATPase, α-Subunit (1019-1034), rabbit polyclonal, recognizes the α-subunit of H+,K+-ATPase. It is validated for use in ELISA, WB, ICC, and IHC (paraffin sections).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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119101
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ca, H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the α-subunit of H+,K+-ATPase.

    • Antibody Target Gene Symbol: ATP4A
    • Target Synonym: ATP6A, Atpase, h+/k+ transporting, alpha polypeptide, Gastric H+/K+ ATPase Alpha, H+/K+ ATPASE, HKATPC, RATHKATPC
    • Entrez Gene Name: ATPase, H+/K+ exchanging, alpha polypeptide
    • Hu Entrez ID: 495 (Related Antibodies: 119102)
    • Mu Entrez ID: 11944
    • Rat Entrez ID: 24216

      		•
      Catalogue Number119101
      Brand Family Calbiochem®
      References
      ReferencesSmolka, A. 1992. Biophys. Biochem. Acta 1108, 75.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Paraffin Sections (see comments)
      Application CommentsExhibits some cross-reactivity with Na+, K+-ATPase at working dilution for H+,K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (GVRCCPGSWWDQGLYY) corresponding to amino acids 1019-1034 of porcine H+, K+-ATPase α-subunit
      ImmunogenPorcine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      119101 0

      Documentation

      Anti-H+,K+-ATPase, α-Subunit (1019-1034) Rabbit pAb Certificates of Analysis

      TitleLot Number
      119101

      References

      Reference overview
      Smolka, A. 1992. Biophys. Biochem. Acta 1108, 75.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-January-2010 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Paraffin Sections (see comments)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-subunit of H+,K+-ATPase.
      HostRabbit
      Immunogen speciesPorcine
      Immunogena synthetic peptide (GVRCCPGSWWDQGLYY) corresponding to amino acids 1019-1034 of porcine H+, K+-ATPase α-subunit
      IsotypeIgG
      Speciescanine, human, mouse, rat
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsExhibits some cross-reactivity with Na+, K+-ATPase at working dilution for H+,K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.
      Toxicity Standard Handling
      ReferencesSmolka, A. 1992. Biophys. Biochem. Acta 1108, 75.