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ABE188 Anti-HEXIM1 Antibody

Use Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1.

Anti-HEXIM1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, Chromatin Biology Gene Symbol: CLP-1, MAQ1, HEXIM1, EDG1, HIS1, CLIP1 UniProt Number: O94992
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ABE188
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE188
      DescriptionAnti-HEXIM1 Antibody
      Alternate Names
      • Protein HEXIM1
      • Cardiac lineage protein 1
      • Estrogen down-regulated gene 1 protein
      • Hexamethylene bis-acetamide-inducible protein 1
      • Menage a quatre protein 1
      • hexamethylene-bis-acetamide-inducible transcript 1
      • HMBA-inducible
      • hexamethylene bisacetamide-inducible protein
      Background InformationHEXIM1 is a transcriptional regulator that functions as a general RNA polymerase II transcription inhibitor. HEXIM1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. HEXIM1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. It is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected HEXIM1 in HeLa and A431 cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human HEXIM1.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryExpression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq].
      Gene Symbol
      • CLP-1
      • MAQ1
      • HEXIM1
      • EDG1
      • HIS1
      • CLIP1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

      SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.

      TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.

      INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.

      DOMAIN: The coiled-coil domain mediates oligomerization.

      MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.

      SEQUENCE SIMILARITIES: Belongs to the HEXIM family.
      Molecular Weight~52 kDa observed. An uncharacterized band at ~60 kDa has been observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected HEXIM1 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE188 04053252483134

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies