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371732 Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb

Anti-Gsα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of Gsα-subunit. It is validated for use in Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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371732
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MaRbPolyclonal Antibody

Pricing & Availability

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371732-50UL
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      Description
      OverviewRecognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits.
      Catalogue Number371732
      Brand Family Calbiochem®
      References
      ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Product Information
      FormLiquid
      FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mammals
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      371732-50UL 04055977213324

      Documentation

      Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb Certificates of Analysis

      TitleLot Number
      371732

      References

      Reference overview
      Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.

      Citations

      Title
    • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein.
      HostRabbit
      Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
      IsotypeIgG
      Speciesmammalian
      Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
      FormLiquid
      FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Citation
    • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.
      		•