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NE1015 Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)

This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GFAP Cocktail

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NE1015
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Gp, H, M, Porcine, R, ShMMonoclonal Antibody

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      Description
      OverviewRecognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
      Catalogue NumberNE1015
      Brand Family Calbiochem®
      SynonymsAnti-GFAP Cocktail
      Application Data
      Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain.

      Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
      References
      ReferencesVick, W.W., et al. 1987. Acta. Cytol. 31, 816.
      McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
      Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlAstrocytes or cytoskeletal preparations
      Preservative≤ 0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:1000)
      Frozen Sections (1:1000, see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000, see comments)
      Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
      Application CommentsThis cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified bovine GFAP protein
      ImmunogenBovine
      CloneSMI-22
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Guinea Pig
      • Human
      • Mouse
      • Porcine
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsUpon initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1015-100UL 04055977209853

      Documentation

      Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22) Certificates of Analysis

      TitleLot Number
      NE1015

      References

      Reference overview
      Vick, W.W., et al. 1987. Acta. Cytol. 31, 816.
      McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
      Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      SynonymsAnti-GFAP Cocktail
      ApplicationELISA (1:1000)
      Frozen Sections (1:1000, see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000, see comments)
      Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
      Application Data
      Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain.

      Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
      DescriptionMouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
      BackgroundGlial fibrillary acidic protein (GFAP) is an intermediate filament protein found only in glial cells or cells of glial origin. It can be detected in astrocytes and certain other astroglia in the CNS, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. GFAP is upregulated and expressed at high levels in astrocytes in many damage and disease states. It is also often highly expressed in neural stem cells and many types of brain tumors.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified bovine GFAP protein
      CloneSMI-22
      IsotypeIgG2b
      Speciesbovine, canine, chicken, guinea pig, human, mouse, porcine, rat, sheep
      Positive controlAstrocytes or cytoskeletal preparations
      FormLiquid
      FormulationUndiluted ascites.
      Preservative≤ 0.1% sodium azide
      CommentsThis cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsUpon initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesVick, W.W., et al. 1987. Acta. Cytol. 31, 816.
      McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
      Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.