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ABS1652 Anti-GSTA4 Antibody

Anti-GSTA4 Antibody is an antibody against GSTA4 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-GSTA4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Oxidative Stress Gene Symbol: Gsta4 UniProt Number: P14942
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ABS1652
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IH(P)RbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1652
      DescriptionAnti-GSTA4 Antibody
      Alternate Names
      • Glutathione S-transferase alpha-4
      • Glutathione S-transferase A4
      • Glutathione S-transferase Yk
      • GSTA4
      • GST 8-8
      • GST A4-4
      • GST K
      • GST Yk
      Background InformationGlutathione S-transferase alpha-4 (EC:2.5.1.18; UniProt P14942; also known as Glutathione S-transferase A4, Glutathione S-transferase Yk, GST 8-8, GST A4-4, GST K, GST Yk) is encoded by the Gsta4 gene (Gene ID 300850) in rat. Glutathione S-transferases constitute a family of isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist either as homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as opposed to the X and Z fractions) that were found during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes, namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma (GSTS), theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTA4 exists as a homodimer composed of two Yk subunits.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GSTA4 Antibody is an antibody against GSTA4 for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GSTA4 in rat kidney, rat liver, and mouse cerebral cortex tissue.
      Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase alpha-4 in liver cytosolic preparations from mice on high-fat diet (F) when compared with liver samples from mice on regular diet (Meakin, P.J., et al. (2014). Mol. Cell. Biol. 34(17):3305-3320).
      Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase alpha-4 levels in liver homogenates from mice fed on diets supplemented with the antioxidant butylated hydroxyanisol (BHA) (Chanas, S.A., et al. (2002). Biochem. J. 365:405-416).
      Western Blotting Analysis: A representative lot detected purified rat liver GST K (YkYk) and kidney GST J (YkYk), but not Ya, Yc, Yb1, Yb2, Yb3, Yf, or Yn subunits from other forms of purified rat GSTs (Hayes, J.D,, and Mantle, T.J. (1986). Biochem. J. 233(3):779-788).
      Biological Information
      ImmunogenPurified rat liver GST K (YkYk).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antiserum selectively reacts with Yk from purified rat liver GST K and rat kidney GST J. No reactivity toward Ya, Yc, Yb1, Yb2, Yb3, Yf, or Yn subunits from other forms of purified rat GSTs (Hayes, J.D,, and Mantle, T.J. (1986). Biochem. J. 233(3):779-788).
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gsta4
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~25 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat kidney tissue lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTA4 in 10 µg of rat kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1652 04055977312508