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MABN2512-25UG Anti-GABA(A) receptor subunit α-1 Antibody, clone 1F4

Anti-GABA(A) receptor subunit -1, clone 1F4, Cat. No. MABN2512, is a mouse monoclonal antibody that detects GABA(A) receptor subunit -1 and is tested for use in Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry, and Radioimmunoassay.

MABN2512-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2512-25UG
      DescriptionAnti-GABA(A) receptor subunit α-1 Antibody, clone 1F4
      Alternate Names
      • Gamma-aminobutyric acid receptor subunit alpha-1
      • GABA A receptor alpha-1
      Background InformationGamma-aminobutyric acid receptor subunit 1 (UniProt: P14867; also known as GABA(A) receptor subunit alpha-1) is encoded by the GABRA1 gene (Gene ID: 2554) in human. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. On binding of two GABA molecules to the extracellular receptor domain, a conformational change is induced in the oligomer and Cl moves into the cell leading to an inhibitory hyperpolarization. GABAA and GABAB receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABA 1 receptor subunit is part of the heteropentamer formed by a combination of 1, β2, and 2 subunits. GABA receptor 1 subunit is synthesized with a signal peptide (aa 1-29) which is subsequently cleaved off to generate the mature form that contains four transmembrane domains (aa 252-273; 279-300; 313-334; and 422-443), one extracellular domain (aa 28-251), and a cytoplasmic domain (aa 335-421). (Ref.: Zhu, S., et al. (2018). Nature. 559(7712); 67-72; Goetz, T., et al. (2007). Prog. Brain Res. 160; 21-41).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GABA(A) receptor subunit -1, clone 1F4, Cat. No. MABN2512, is a mouse monoclonal antibody that detects GABA(A) receptor subunit -1 and is tested for use in Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry, and Radioimmunoassay.
      Key Applications
      • Immunocytochemistry
      • Radioimmunoassay
      • ELISA
      • Electron Microscopy
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected GABA(A) receptor subunit -1 in SH-SY5Y cells.

      Radioimmunoassay: A representative lot detected GABA(A) receptor subunit -1 in Radioimmunoassay applications (Zhu, S., et. al. (2018). Nature. 559(7712):67-72).

      ELISA Analysis: A representative lot detected GABA(A) receptor subunit -1 in ELISA applications (Zhu, S., et. al. (2018). Nature. 559(7712):67-72).

      Electron Microscopy: A representative lot detected GABA(A) receptor subunit -1 in Electron Microscopy applications (Kim, J.J., et. al. (2020). Nature. 585(7824); 303-308).

      ).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHuman pentameric ( 1β2 2) GABA-A receptor protein.
      EpitopeUnknown
      Clone1F4
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1F4 is a mouse monoclonal antibody that detects GABA receptor -1.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GABRA1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight51.80 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in SK-N-SH cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected GABA(A) receptor subunit -1, in SK-N-SH cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2512-25UG 04065265324274