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MABC1021 Anti-G-CSF-R/CD114 Antibody, clone 5A11.1

This Anti-G-CSF-R Antibody, clone 5A11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of CD114.

Anti-G-CSF-R/CD114 Antibody, clone 5A11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: CSF3R, GCSFR UniProt Number: Q99062
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MABC1021
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1021
      DescriptionAnti-G-CSF-R/CD114 Antibody, clone 5A11.1
      Alternate Names
      • Granulocyte colony-stimulating factor receptor
      • CD114
      • G-CSF-R
      • G-CSF receptor
      Background InformationGranulocyte colony-stimulating factor receptor (UniProt Q99062; also known as CD114, G-CSF-R, G-CSF receptor) is encoded by the CSF3R (also known as GCSFR) gene (Gene ID 1441) in human. CD114/G-CSF-R belongs to the type I subgroup of the superfamily of cytokine receptors. G-CSF-R mediates granulocyte proliferation and differentiation in both normal and malignant hematopoiesis. G-CSF-R functions as the receptor for granulocyte colony-stimulating factor (G-CSF) and is activated via ligand-induced homodimerization. The G-CSF/GCSF-R complex mediates survival, proliferation, and maturation of neutrophils and regulates granulopoiesis under both normal and stress conditions. Cell signaling via G-CSF-R is transduced primarily via the Jak/STAT pathway, but the Ras/Raf/MAP kinase and PKB/Akt pathways are also involved. CSF3R gene mutations are well documented in congenital neutropenia as well as the associated predisposition to myelodysplasia and acute myeloid leukemia myeloproliferative disorders. Human G-CSF-R is produced with a signal peptide sequence (a.a. 1-24), the removal of which yields the mature single transmembrane (a.a. 628-650) receptor with a C-terminal cytoplasmic tail (a.a. 651-836) and a large N-terminal extracellular sequence (a.a. 25-627) composed of a C2-type Ig-like domain (a.a. 25-117) and five type-III fibronectin domains (a.a. 125-623).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-G-CSF-R Antibody, clone 5A11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of CD114.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected G-CSF-R/CD114 in human placenta and bone marrow tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human G-CSF-R/CD114 cytoplasmic domain fragment.
      EpitopeCytoplasmic domain.
      Clone5A11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5A11.1 was raised against a cytoplasmic domain fragment of the canonical isoform GCSFR-1. Cross-reactivity of this monoclonal antibody toward spliced isoforms GCSFR-2, -3, and -4 is likely, but has not been confirmed.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CSF3R
      • GCSFR
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~43.2 kDa observed for fragment. 89.62 kDa (isoform GCSFR-1), 82.58 kDa (isoform GCSFR-2), 92.58 kDa (isoform GCSFR-3), 84.07 kDa (isoform GCSFR-4; D7) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting of GST-tagged human G-CSF-R cytoplasmic domain fragment.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected 50 ng of GST-tagged recombinant human G-CSF-R cytoplasmic domain fragment.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1021 04055977355321