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MAB2262 Anti-Excitatory amino acid transporter 2 Antibody, clone G6

This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: SLC1A2, EAAT2, GLT-1, GLT1 UniProt Number: P43004
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MAB2262
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2262
      DescriptionAnti-Excitatory amino acid transporter 2 Antibody, clone G6
      Alternate Names
      • Glutamate/aspartate transporter II
      • Sodium-dependent glutamate/aspartate transporter 2
      • Solute carrier family 1 member 2
      • excitatory amino acid transporter 2
      • excitotoxic amino acid transporter 2
      • glial high affinity glutamate transporter
      • solute carrier family 1 (glial high affinity glutamate transporter), member 2
      • solute carrier family 1, member 2
      Background InformationThe activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Mouse brain tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 0.5 µg/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 µg of human brain tissue lysate.
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2.
      EpitopeC-terminus
      CloneG6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the C-terminus of Excitatory amino acid transporter 2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rat based on 100% immunogen peptide sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
      Gene Symbol
      • SLC1A2
      • EAAT2
      • GLT-1
      • GLT1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

      SUBUNIT STRUCTURE: Homotrimer. Interacts with JUB By similarity.

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

      PTM: Glycosylated.

      SEQUENCE SIMILARITIES: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. [View classification]
      Molecular Weight62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected Excitatory amino acid transporter 2 on 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2262 04053252467424

      Documentation

      Anti-Excitatory amino acid transporter 2 Antibody, clone G6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Excitatory amino acid transporter 2 Antibody, clone G6 Certificates of Analysis

      TitleLot Number
      Anti-Excitatory amino acid transporter 2, -2736601 2736601
      Anti-Excitatory amino acid transporter 2, clone G6 - 2120072 2120072
      Anti-Excitatory amino acid transporter 2, clone G6 - 2388341 2388341
      Anti-Excitatory amino acid transporter 2, clone G6 - 2430438 2430438
      Anti-Excitatory amino acid transporter 2, clone G6 - 2455734 2455734
      Anti-Excitatory amino acid transporter 2, clone G6 - 2015589 2015589
      Anti-Excitatory amino acid transporter 2, clone G6 - 2197288 2197288
      Anti-Excitatory amino acid transporter 2, clone G6 - 2280474 2280474
      Anti-Excitatory amino acid transporter 2, clone G6 - 3063386 3063386
      Anti-Excitatory amino acid transporter 2, clone G6 - 3284348 3284348
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies