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GR01 Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studi

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Anti-EGFR (Ab-1) Mouse mAb (528) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epidermal Growth Factor Receptor

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GR01
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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GR01-100UG
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      Description
      OverviewRecognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.
      Catalogue NumberGR01
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor
      References
      ReferencesHunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlHL-60 cells or human brain
      Positive controlA-431 or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation(1 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (see application references)
      Application CommentsThis antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      Epitopewithin the extracellular domain
      Clone528
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      GR01-100UG 04055977220162