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MABT535-25UG Anti-Desmin Antibody, clone 4C12.1

Anti-Desmin Antibody, clone 4C12.1, Cat. No. MABT535, is a mouse monoclonal antibody that targets Desmin in Human, Mouse, and Rat and it is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Desmin Antibody, clone 4C12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: DES UniProt Number: P17661
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MABT535-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT535-25UG
      DescriptionAnti-Desmin Antibody, clone 4C12.1
      Background InformationDesmin (UniProt: P17661) is encoded by the DES gene (Gene ID: 1674) in human. Desmin is a major class III intermediate filament protein essential for the structural integrity and function of muscle.
      In adult striated muscle desmin forms a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. It may also act as a sarcomeric microtubule-anchoring protein and specifically associate with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Desmin is particularly abundant at the myotendinous junctions and at the neuromuscular junctions of skeletal muscle. Mammalian cardiac muscle cells contain higher amounts of desmin (up to 2% of total protein) compared to skeletal muscle cells (0.35% of total protein). Mutations in DES gene have been linked to familial cardiac and skeletal myopathy characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, and restrictive heart failure. It can also lead to myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. (rEF.: Denise Paulin, D., and Li, Z (2004). Experimental Cell Research 301(1); 1 -7).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Desmin Antibody, clone 4C12.1, Cat. No. MABT535, is a mouse monoclonal antibody that targets Desmin in Human, Mouse, and Rat and it is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Desmin in mouse heart, mouse skeletal muscle, rat heart, and rat skeletal muscle tissues.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmin in human heart and human skeletal muscle tissues.
      Biological Information
      ImmunogenGST/His-taged recombinant fragment corresponding to 64 amino acids from the C-terminal region of human Desmin.
      Clone4C12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4C12.1 specifically detects Desmin in human, mouse, and rat muscles. It targets an epitope within 64 amino acids from the C-terminal end.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • DES
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~53 kDa observed; 53.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmin in 10 µg of human skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT535-25UG 04054839553615