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MABS2291-25UL Anti-DPP4/CD26 Antibody, clone KU44.13A

This Anti-DPP4/CD26, clone KU44.13A, Cat. No. MABS2291, is a mouse monoclonal antibody that detects DPP4/CD26 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

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MABS2291-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2291-25UL
      DescriptionAnti-DPP4/CD26 Antibody, clone KU44.13A
      Alternate Names
      • Dipeptidyl peptidase 4
      • EC:3.4.14.5
      • ADABP
      • Adenosine deaminase complexing protein 2
      • ADCP-2
      • Dipeptidyl peptidase IV
      • DPPIV
      • T-cell activation antigen CD26
      • TP103
      • CD26
      Background InformationDipeptidyl peptidase 4 (UniProt: P27487; also known as EC:3.4.14.5, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD26) is encoded by the DPP4 (also known as CD26, ADCP2) gene (Gene ID: 1803) in human. DPP4 is a single-pass type II membrane glycoprotein that is ubiquitously expressed on the surface of a variety of cells. It is expressed in endothelial cells, fibroblasts, and lymphocytes and on the apical surfaces of epithelial and acinar cells. It serves as a serine exopeptidase that cleaves X-proline or X-alanine dipeptides from the N-terminus of polypeptides. A soluble form of DPP4 is also present (aa 39-766) that is derived from the membrane by proteolytic processing. DPP4 expression is substantially dysregulated in a variety of disease states including inflammation, cancer, obesity, and diabetes and its inhibition by the gliptin family of drugs has gained importance for the therapy of type 2 diabetes. DPP4 is also involved in the costimulatory signal that is essential for T-cell receptor (TCR)-mediated T-cell activation. Its levels are up-regulated by IL-12 on activated T-cells and down-regulated by TNF. DPP4 also plays a role in tumor development and its effect depends on the tumor type and its microenvironment. It has a tumor suppressor effect in several cancers including melanoma, ovarian cancer, non-small cell lung cancer, prostate cancer, endometrial cancer, and neuroblastoma. Higher levels of DPP4 have been observed in pancreatic cancer and knockdown of DPP4 expression inhibits cell growth, migration, and invasion. (Ref.: Arias-Pinilla, GA., et al. (2020). Sci. Rep. 10; 537; Röhrborn, D., et al. (2015). Front. Immunol. 6; 386).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis Anti-DPP4/CD26, clone KU44.13A, Cat. No. MABS2291, is a mouse monoclonal antibody that detects DPP4/CD26 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • ELISA
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected DPP4/CD26 in Immunofluorescence applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated DPP4/CD26 in Immunoprecipitation applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep.10(1):537).

      ELISA Analysis: A representative lot detected DPP4/CD26 in ELISA applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

      Flow Cytometry Analysis: A representative lot detected DPP4/CD26 in Flow Cytometry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

      Immunohistochemistry Applications: A representative lot detected DPP4/CD26 in Immunohistochemistry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenCFPAC-1 human pancreatic cancer cells and SP2 myeloma cells.
      EpitopeExtracellular domain
      CloneKU44.13A
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone KU44.13A is a mouse monoclonal antibody that detects Dipeptidyl peptidase 4 (DPP4)/CD26.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DPP4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight88.28 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HPAF-II cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected DPP4/CD26 in one million HPAF-II cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2291-25UL 04065267027654