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MABE425 Anti-DOT1L Antibody, clone 1A11.2

This Anti-DOT1L Antibody, clone 1A11.2 is validated for use in Western Blotting for the detection of DOT1L.

Anti-DOT1L Antibody, clone 1A11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: DOT1L, KIAA1814, KMT4 UniProt Number: Q8TEK3
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MABE425
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE425
      DescriptionAnti-DOT1L Antibody, clone 1A11.2
      Alternate Names
      • Histone-lysine N-methyltransferase, H3 lysine-79 specific
      • Disruptor of telomeric silencing-1 like
      • DOT1-like protein
      • H3-K79-HMTase
      • Histone H3-K79 methyltransferase
      • Lysine N-methyltransferase 4
      Background InformationHistone-lysine N-methyltransferase, H3 lysine-79 specific (EC 2.1.1.43; UniProt Q8TEK3; also known as Disruptor of telomeric silencing-1 like, DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4) is encoded by the DOT1L (also known as KIAA1814, KMT4) gene (Gene ID 84444) in human. Disruptor of telomeric silencing-1 like (DOT1L) catalyzes the mono-, di-, and tri-methylation of histone H3 on lysine-79 (H3K79). DOT1L-mediated H3K79 methylation is associated with active transcription. Accumulating evidences indicate a role of DOT1L and H3K79 in many biological processes, including DNA damage response, cell cycle, embryonic cell development, as well as the initiation and maintenance of mixed lineage leukemia (MLL)-rearranged leukemia. MLL fusion proteins such as AF9, AF10, and ENL recruit DOT1L to their target gene loci to help promote the transcription of genes that contribute to leukemic transformation of hematopoietic progenitor cells. DOT1L also has been identified as a coactivator of Wnt signaling in colorectal cancer, and is shown to induce neoplastic transformation of immortalized human breast epithelial cells and increase tumor initiation and growth of human breast cancer cells. In addtion, DOT1L is reported to regulate androgen receptor activity via direct methylation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DOT1L Antibody, clone 1A11.2 is validated for use in Western Blotting for the detection of DOT1L.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human DOT1L.
      EpitopeInternal (N-terminal half).
      Clone1A11.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1A11.2 detects both spliced isoforms of human DOT1L reported by UniProt (Q8TEK3).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DOT1L
      • KIAA1814
      • KMT4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~185/165 kDa observed. 164.9 kDa (isoform 1; Q8TEK3-2) and 184.9 kDa (isoform 2; Q8TEK3-1) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat nuclear extract.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected DOT1L isoforms in 10 µg of Jurkat nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE425 04055977353549