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MABE1095 Anti-DNA-RNA Hybrid Antibody, clone S9.6

Anti-DNA-RNA Hybrid, clone S9.6, Cat. No. MABE1095, is a highly specific mouse monoclonal antibody, that targets DNA-RNA hybrid and has been tested in Affinity Binding Assay, Chromatin Immunoprecipitation (ChIP), ChIP-seq, Dot Blot, Immunocytochemistry, and Immunoprecipitation.

Anti-DNA-RNA Hybrid Antibody, clone S9.6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function
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MABE1095
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AABA, ChIP, DB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1095
      DescriptionAnti-DNA-RNA Hybrid Antibody, clone S9.6
      Alternate Names
      • DNA-RNA Duplex
      • DNA/RNA Duplex
      • DNA:RNA Duplex
      • DNA-RNA Hybrid
      • DNA/RNA Hybrid
      • DNA:RNA Hybrid
      • RNA-DNA Duplex
      • RNA/DNA Duplex
      • RNA:DNA Duplex
      • RNA-DNA Hybrid
      • RNA/DNA Hybrid
      • RNA:DNA Hybrid
      Background InformationDNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DNA-RNA Hybrid, clone S9.6, Cat. No. MABE1095, is a highly specific mouse monoclonal antibody, that targets DNA-RNA hybrid and has been tested in Affinity Binding Assay, Chromatin Immunoprecipitation (ChIP), ChIP-seq, Dot Blot, Immunocytochemistry, and Immunoprecipitation.
      Key Applications
      • Affinity Binding Assay
      • Chromatin Immunoprecipitation (ChIP)
      • Dot Blot
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesDot Blot Analysis: 0.2 µg/mL from a representative lot detected an enhanced level of DNA-RNA hybrids in genomic extracts from RNase H-deficient yeast strain than extracts from wild-type strain (Courtesy of Lorenzo Costantino, Ph.D., Koshland Lab, University of California at Berkley, U.S.A.).

      Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805).

      Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716).

      Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).
      Biological Information
      ImmunogenDNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
      CloneS9.6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Species Reactivity NoteAll. Target DNA-RNA heteroduplex (R loop) structure is not sequence- or species-specific.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody immunolocalized nuclear and mitochondrial DNA-RNA hybrids in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1095 04054839114496

      Documentation

      Anti-DNA-RNA Hybrid Antibody, clone S9.6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DNA-RNA Hybrid Antibody, clone S9.6 Certificates of Analysis

      TitleLot Number
      Anti-DNA-RNA Hybrid, clone S9.6 - 3152139 3152139
      Anti-DNA-RNA Hybrid, clone S9.6 - 3172456 3172456
      Anti-DNA-RNA Hybrid, clone S9.6 - 3224659 3224659
      Anti-DNA-RNA Hybrid, clone S9.6 - 3240433 3240433
      Anti-DNA-RNA Hybrid, clone S9.6 - 3275033 3275033
      Anti-DNA-RNA Hybrid, clone S9.6 - 3328189 3328189
      Anti-DNA-RNA Hybrid, clone S9.6 - 3474102 3474102
      Anti-DNA-RNA Hybrid, clone S9.6 - 3523210 3523210
      Anti-DNA-RNA Hybrid, clone S9.6 - 3577422 3577422
      Anti-DNA-RNA Hybrid, clone S9.6 - 3587102 3587102
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