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MABC1798-100UL Anti-Claudin-6 Antibody, clone 2

Anti-Claudin-6, clone 2, Cat. No. MABC1798, is a mouse monoclonal antibody that detects Claudin-6 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Inhibition Assay, and Western Blotting.

MABC1798-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1798-100UL
      DescriptionAnti-Claudin-6 Antibody, clone 2
      Alternate Names
      • Skullin
      Background InformationClaudin-6 (UniProt: P56747; also known as Skullin) is encoded by the CLDN6 (also known as UNQ757/PRO1488) gene (Gene ID: 9074) in human. Claudin-6 is a multi-pass membrane protein that plays a major role in tight junction-specific obliteration of the intercellular space. Claudin-6 has two extracellular domains (aa 29-81 and 138-160), four transmembrane domains (aa 8-28, 82-102, 117-137, and 161-181), and three cytoplasmic domains (aa 1-7; 103-116, and 182-220). It acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells. Claudin-6 is expressed in the liver, in peripheral blood mononuclear cells, and hepatocarcinoma cell lines. It is also expressed specifically in undifferentiated stem cells and can serve as a marker to purify stem-like cells from a mixed cell population. It is shown to play an important role during mouse embryonic epithelium formation and the development of endodermal tissues, and its level of expression is correlated with dedifferentiation and malignant proliferation of epithelial cells. (Ref.: Kong, F-E. et al. (2021). Sci. Transl. Med. 13, eabb6282; Wang, L., et al. (2012). Cell Res. 22; 1082-1085; Zheng, A., et al. (2007). J. Virol. 81(22); 12465-12471).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Claudin-6, clone 2, Cat. No. MABC1798, is a mouse monoclonal antibody that detects Claudin-6 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Inhibition
      • Flow Cytometry
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesTested applications

      Immunocytochemistry Analysis: A representative lot detected Claudin-6 in Immunocytochemistry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

      Inhibition Assay: A representative lot inhibited tumor growth of HepG2 xenograft tumors in a murine model. (Kong, F-E., et al. (2021). Sci Transl Med.;13(579):eabb6282).

      Immunohistochemistry Applications: A representative lot detected Claudin-6 in Immunohistochemistry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

      Western Blotting Analysis: A representative lot detected Claudin-6 in Western Blotting applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

      Flow Cytometry Analysis: A representative lot detected Claudin-6 in Flow Cytometry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenExtracellular peptides specific for human Claudin-6.
      EpitopeExtracellular domain
      Clone2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2 is a mouse monoclonal antibody that detects Claudin-6. It targets an epitope within the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLDN6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~27 kDa observed; 23.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NTERA-2 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Claudin-6 in NTERA-2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1798-100UL 04065270172464