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ABT45 Anti-Claudin-5 Antibody

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ABT45
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT45
      DescriptionAnti-Claudin-5 Antibody
      Alternate Names
      • Claudin-5
      • Transmembrane protein deleted in VCFS
      • TMDVCF
      Background InformationClaudin-5 (UniProt: O00501; also known as Transmembrane protein deleted in VCFS, TMDVCF) is encoded by the CLDN5 (also known as AWAL, TMVCF) gene (Gene ID: 7122) in human. Claudin-5 is a multi-pass membrane protein with three cytoplasmic domains (aa 1-7; 103-122; and 181-218); four transmembrane domains (aa 8-28, 82-102, 123-143, and 160-180); and two extracellular loops (aa 29-81 and 144-159). It plays a major role in tight junction-specific obliteration of the intercellular space. It is localized specifically to the endothelial cell layer in the brain, lung, liver, kidney, and skin. In peripheral tissues, it displays greater heterogeneity in tissue expression. At the blood-brain barrier, it is one of the most enriched tight junction proteins and its dysfunction has been linked to several neurodegenerative diseases, including Alzheimer s disease and multiple sclerosis. Cldn5-null mice are reported to die shortly after birth. (Ref.: Hashimoto, Y., et al. (2018). Sci. Rep. 8; 8383; Greene, C., et al. (2019). Fluids Barriers CNS. 16;3).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Human lung tissue lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Claudin-5, Cat. No. ABT45, is a rabbit polyclonal antibody that detects Claudin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Claudin-5 in Mouse and Rat Lung tissue lysate.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Claudin-5 in human prostate tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Claudin-5.
      EpitopeCytoplasmic Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Claudin-5. It targets an epitope within 12 amino acids from the C-terminal region.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Horse, Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • CLDN5
      • AWAL
      • TMVCF
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space (By similarity).

      SUBUNIT STRUCTURE: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).

      SUBCELLULAR LOCATION: Cell junction › tight junction. Cell membrane; Multi-pass membrane protein.

      SEQUENCE SIMILARITIES: Belongs to the claudin family.
      Molecular Weight~17 kDa observed; 23.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human Lung tissue lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-5 in Human lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT45 04053252556180

      Documentation

      Anti-Claudin-5 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Claudin-5 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Claudin-5 (rabbit polyclonal) Polyclonal Antibody Q2925822
      Anti-Claudin-5 - 2343143 2343143
      Anti-Claudin-5 - 2424829 2424829
      Anti-Claudin-5 - 2464635 2464635
      Anti-Claudin-5 - 2060504 2060504
      Anti-Claudin-5 - 2181289 2181289
      Anti-Claudin-5 - 2211525 2211525
      Anti-Claudin-5 - 2280481 2280481
      Anti-Claudin-5 - 3243369 3243369
      Anti-Claudin-5 - 3439722 3439722

      References

      Reference overviewApplicationPub Med ID
      Bacterial induction of Snail1 contributes to blood-brain barrier disruption.
      Kim, BJ; Hancock, BM; Bermudez, A; Del Cid, N; Reyes, E; van Sorge, NM; Lauth, X; Smurthwaite, CA; Hilton, BJ; Stotland, A; Banerjee, A; Buchanan, J; Wolkowicz, R; Traver, D; Doran, KS
      The Journal of clinical investigation  125  2473-83  2015

      Show Abstract
      25961453 25961453
      Disrupted tight junctions in the small intestine of cystic fibrosis mice.
      De Lisle, RC
      Cell and tissue research  355  131-42  2014

      Show Abstract
      24169862 24169862
      Cranial irradiation alters the brain's microenvironment and permits CCR2+ macrophage infiltration.
      Morganti, JM; Jopson, TD; Liu, S; Gupta, N; Rosi, S
      PloS one  9  e93650  2014

      Show Abstract
      Western Blotting24695541 24695541