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ABN458 Anti-Choline Transporter Antibody

Anti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Choline Transporter Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SLC5A7, CHT1 UniProt Number: Q9GZV3
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ABN458
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN458
      ReplacesAB5960;AB5966
      DescriptionAnti-Choline Transporter Antibody
      Alternate Names
      • High affinity choline transporter 1
      • Hemicholinium-3-sensitive choline transporter
      • CHT
      • Solute carrier family 5 member 7
      Background InformationHigh affinity choline transporter 1 (UniProt: Q9GZV3; also known as Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7) is encoded by the SLC5A7 (also known as CHT1) gene (Gene ID: 60482) in human. CHT is a multi-pass membrane protein with six extracellular domains, eleven transmembrane domains, and six cytoplasmic domains. It is expressed in cholinergic neurons in putamen, spinal cord, and medulla and serves as a transmembrane transporter that imports choline from the extracellular space into neurons with high affinity in a sodium and chloride ion-dependent manner. In neurons it is acetylated by the action of choline acetyltransferase to generate acetylcholine. Choline uptake is shown to be the rate-limiting step in acetylcholine synthesis. The majority of CHT is localized intracellularly including a proportion present on acetylcholine-containing synaptic vesicles. Diminished levels of acetylcholine in selected areas of the nervous system are linked to several neurodegenerative diseases. Mutation in SLC5A7 gene have been linked to neuronopathy and myasthenic syndrome that is characterized by failure of neuromuscular transmission, fatigability, and muscle atrophy and weakness. (Ref.: Bauche, S., et al. (2016). Am. J. Hum. Genet. 99(3); 753-761; Barwick, KES., et al. (2012). Am. J. Hum. Genet. 91(6); 1103-1107).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 77 amino acids from the C-terminal region of human High affinity choline transporter.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects high affinity choline transporter. It targets an epitope within the C-terminal region.
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC5A7
      • CHT1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~63 kDa observed; 63.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat spinal cord tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Choline Transporter in Rat spinal cord tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN458 04053252941436

      Documentation

      Anti-Choline Transporter Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Choline Transporter Antibody Certificates of Analysis

      TitleLot Number
      Anti-Choline Transporter - Q2343010 Q2343010
      Anti-Choline Transporter - 3218873 3218873
      Anti-Choline Transporter - 3288744 3288744
      Anti-Choline Transporter - 3474173 3474173
      Anti-Choline Transporter - 3745922 3745922
      Anti-Choline Transporter - 3783265 3783265
      Anti-Choline Transporter - 3857741 3857741
      Anti-Choline Transporter - 3861356 3861356
      Anti-Choline Transporter - 3956639 3956639
      Anti-Choline Transporter - 4050752 4050752
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