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ABF1168-100UL Anti-Chitinase-1 (CHIT1)

Anti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Chitinase-1 (CHIT1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CHIT1 UniProt Number: Q13231
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ABF1168-100UL
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF1168-100UL
      DescriptionAnti-Chitinase-1 (CHIT1)
      Alternate Names
      • Chitotriosidase-1
      • EC: 3.2.1.14
      • Chitinase-1
      Background InformationChitotriosidase-1 (UniProt: Q13231; also known as EC: 3.2.1.14, Chitinase-1) is encoded by the CHIT1 gene (Gene ID: 1118) in human. Chitinase-1 is a member of the Chitinase class II subfamily that degrades chitin, chitotriose and chitobiose. It is one of the most abundantly secreted protein and is mainly produced by activated macrophages and epithelial cells. Chitinase -1 can hydrolyze the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. It may also participate in the defense against nematodes and other pathogens. Four isoforms of Chitinase-1 have been described that are produced by alternative splicing and isoform 3, which lacks amino acids 344-372, does not possess any enzymatic activity. Chitinase-1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved to generate the mature form. Its chitin-binding domain is located in amino acids 417-466 and has three chitooligosaccharide binding regions (aa 7-71; 97-100; and 210-213). Elevated levels of Chitinase-1 are observed in cases of Gaucher's disease, bronchial asthma, and atherosclerosis. (Ref.: Kanneganti, M., et al. (2012). J. Epithel. Biol. Pharmacol. 5; 1-9).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Chitinase-1 (CHIT1) in human spleen and bone marrow tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chitinase-1.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Chitinase-1 in human serum. It targets an epitope within 15 amino acids from the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHIT1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~ 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human serum.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Chitinase-1 in human serum.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF1168-100UL 04054839448119