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MAB10753 Anti-Caspase3 (active form) Antibody, clone 3D9.3

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CASP3, CPP32 UniProt Number: P42574
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MAB10753
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB10753
      Replaces04-1090; 04-439
      DescriptionAnti-Caspase3 (active form) Antibody, clone 3D9.3
      Alternate Names
      • Caspase-3
      • CASP-3
      • Apopain
      • Cysteine protease CPP32
      • CPP-32
      • Protein Yama
      • SREBP cleavage activity 1
      • SCA-1
      • Caspase-3 subunit p17
      • Caspase-3 subunit p12
      Background InformationCaspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
      Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Caspase3 (active form).
      Clone3D9.3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CASP3
      • CPP32
      UniProt Number
      Molecular Weight~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB10753 04053252850653