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MABC1593-100UG Anti-CRBN Antibody, clone 4D6

Anti-CRBN, clone 4D6 , Cat. No. MABC1593, is a mouse monoclonal antibody that detects Cereblon and has been tested for use in Immunoprecipitation and Western Blotting.

MABC1593-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1593-100UG
      DescriptionAnti-CRBN Antibody, clone 4D6
      Alternate Names
      • Protein Cereblon
      • CRBN
      Background InformationProtein cereblon (UniProt: Q96SW2) is encoded by the CRBN (also known s AD-006) gene (Gene ID: 51185) in human. Cereblon is a widely expressed peripheral membrane protein that serves as a substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (RBX1) and the adaptor protein damaged DNA-binding protein 1 (DDB1) to form a CRL4 E3 complex, where RBX1 serves as the docking site for the activated E2 protein, and DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex. Cereblon has been recognized as the primary teratogenic target of thalidomide and its analogs. These drugs exert their antiproliferative effects on multiple myeloma cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation. Human cereblon is a 442-amino acid protein composed of a Lon protease-like scaffold (aa 80-317) with a helical insertion that mediates DDB1 binding and a thalidomide-binding domain (aa 339-442).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CRBN, clone 4D6 , Cat. No. MABC1593, is a mouse monoclonal antibody that detects Cereblon and has been tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: 20 ug from a representative lot detected CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).
      Biological Information
      ImmunogenHis-tagged recombinant protein fragment corresponding to the N-terminal region of human Cereblon.
      Clone4D6 (-3H10-D6)
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4D6 (-3H10-D6) is a mouse monoclonal antibody that detects human Cereblon. It targets an epitope within the N-terminal domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRBN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MOLT-4 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Cereblon.(CRBN) in MOLT-4 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1593-100UG 04061841039143