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MABT1366-25UG Anti-CRB3A Antibody, clone 1E6

Anti-CRB3A, clone 1E6, Cat. No. MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABT1366-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1366-25UG
      DescriptionAnti-CRB3A Antibody, clone 1E6
      Alternate Names
      • Protein crumbs homolog 3
      Background InformationProtein crumbs homolog 3 (UniProt: Q9BUF7) is encoded by the CRB3 (also known as UNQ588/PRO1158) gene (Gene ID: 92359) in human. Mammalian cells contain three Crb orthologs. CRB1 is primarily expressed in the brain, cornea, and retina. CRB2 is expressed in lung, kidney, retina, colon and mammary glands. It is also expressed in at moderate levels in Expressed at moderate levels in liver, spleen, pancreas, placenta, and prostate tissue. CRB3 is present in epithelial tissues and is present in two isoforms, CRB3A and CRB3B. CRB3 is a single-pass type I membrane protein that is reported to control epithelial cell polarization. CRB3A is apically localized and promotes apical membrane growth and is and is essential for the formation of tight junctions in epithelial cells. It also prevents the epithelial-to-mesenchymal transition (EMT), which is known to contribute to tumor progression. CRB3 is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 27-59), a transmembrane domain (aa 60-80), and a cytoplasmic tail (aa 81-120), which contains a PDZ-domain binding motif (aa 117-120) that interacts with the Maguk protein, PALS1, which recruits PALS1-associated tight junction (PATJ) protein into the CRB3A complex. Both PALS1 and PATJ play a critical role in epithelial cell polarity and tight junction formation. CRB3A is shown to control and properly localize RhoA signaling that is required for CRB3A-dependent reorganization of the actin cytoskeleton. (Ref.: Loie, E., et al (2015). Mol. Cell Biol. 35(19); 3423-3435).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CRB3A, clone 1E6, Cat. No. MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).

      Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
      EpitopeC-terminus
      Clone1E6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Drosophila, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRB3
      • UNQ588
      • PRO1158
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 12.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CRB3A in human kidney tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1366-25UG 04054839613814

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