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MABT1562-25UL Anti-CFAP43 Antibody, clone 27D5

Anti-CFAP43, clone 27D5, Cat. No. MABT1562, is a mouse monoclonal antibody that detects CFAP43 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABT1562-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1562-25UL
      DescriptionAnti-CFAP43 Antibody, clone 27D5
      Alternate Names
      • Cilia- and flagella-associated protein 43
      Background InformationCilia- and flagella-associated protein 43 (UniProt: E9Q7R9; also known as CFAP43) is encoded by the Cfap43 gene (Gene ID: 100048534) in murine species. CFAP43, a flagellar protein with six WD repeats, is expressed in testis, lung, brain, oviduct, and nasal cavity. It is detected very early during embryonic development It is involved in the regulation of the beating frequency of motile cilia on the epithelial cells. It is also involved in the formation of flagellar axoneme organization and function during spermatogenesis. Mutations in Cfap43 gene have been linked to male infertility. Any malfunction of motile cilia, resulting from defective CFAP43, cause a variety of developmental defects in humans and animal models. These defects may also include impaired mucociliary clearance of airways and hydrocephalus. Cfap43 knockout mice (-/-) exhibit dilation of the third and lateral ventricles. (Ref.: Rachev, E. (2020). Dev. Biol. 459(2); 109-125; Morimoto, Y., et al. (2019). Neurology. 92(20); e2364-e2374; Coutton, C., et al. (2018). Nature Commun. 9; Article 686).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-CFAP43, clone 27D5, Cat. No. MABT1562, is a mouse monoclonal antibody that detects CFAP43 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected CFAP43 in Western Blotting applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

      Immunofluorescence Analysis: A representative lot detected CFAP43 in Immunofluorescence applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CFAP43 in Immunoprecipitation applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenMBP-tagged recombinant fragment corresponding to 174 amino acids from the C-terminal half of mouse Cilia- and flagella-associated protein 43 (CFAP43).
      EpitopeC-terminal half
      Clone27D5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 27D5 is a mouse monoclonal antibody that detects Cilia- and flagella-associated protein 43. It targets an epitope within the C-terminal half.
      IsotypeIgG2b
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cfap43
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~190 kDa observed; 193.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse testis tissue extracts.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP43 in Mouse testis tissue extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1562-25UL 04065269135104