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MABF299 Anti-CCR2 Antibody, clone 8C3.1

This mouse monoclonal Anti-CCR2 Antibody, clone 8C3.1, Cat. No. MABF299 is validated for use in Flow Cytometry and Western Blotting for the detection of CCR2.

Anti-CCR2 Antibody, clone 8C3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CCR2, CMKBR2 UniProt Number: P41597
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MABF299
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF299
      DescriptionAnti-CCR2 Antibody, clone 8C3.1
      Alternate Names
      • C-C chemokine receptor type 2
      • C-C CKR-2
      • CC-CKR-2
      • CCR-2
      • CCR2
      • CD192
      • MCP-1-R
      • Monocyte chemoattractant protein 1 receptor
      Background InformationC-C chemokine receptor type 2 (UniProt P41597; also known as C-C CKR-2, CC-CKR-2, CCR-2, CCR2, CD192, MCP-1-R, Monocyte chemoattractant protein 1 receptor) is encoded by the CCR2 (also known as CMKBR2) gene (Gene ID 729230) in human. CC chemokine receptors (CCRs) are 7-transmembrane G protein-coupled receptors (GPCRs) that bind CC chemokines (CCLs) and mediate their biological activities. CCRs and CCLs play a central role in physiological and pathological recruitment of immune cells. CCR2 can interact with CCL2, CCL8 and CCL16, it is found on the surface of monocytes, activated memory T cells, B cells, and basophils in humans, and also in peritoneal macrophages in mice. CCR2 plays an important role in the recruitment of monocytes/macrophages and T cells. CCR2-knockout mice show a partial defect in macrophage function, decreased Th1 immune response, reduced delayed-type hypersensitivity reaction, and are more susceptible to pathogenic bacteria infections. Human CCR2 is a 7-transmembrane (a.a. 43-70, 81-100, 115-136, 154-178, 207-226, 244-268, 286-309) GPCR with an N-terminal extracellular domain (a.a. 1-42) and a C-terminoal cytoplasmic domain (a.a. 310-374).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-CCR2 Antibody, clone 8C3.1, Cat. No. MABF299 is validated for use in Flow Cytometry and Western Blotting for the detection of CCR2.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected surface CCR2 expression among the gated lymphocytes population from one million human PMBCs.
      Biological Information
      ImmunogenGST-tagged recombinant human N-terminal extracellular domain.
      Clone8C3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8C3.1 targets the N-terminal extracelluar domain of human CCR2 spliced isoforms 1&2 reported by Uniprot (P41597).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCR2
      • CMKBR2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~40-50 kDa observed. 41.92 kDa (isoform 1) and 41.06 kDa (isoform 2) calculated. Larger-than-calculated sizes and smeary banding pattern are consistent with the detection of differentially glycosylated CCR2 species. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U937 cell lysate.

      Western Blotting Analysis (WB): A 100,000 dilution of this antibody detected CCR2 in 10 µg of human lymphoma U937 monocyte lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF299 04054839049682