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MABE948 Anti-BRE1-A (RNF20) Antibody, clone 8A4.2

Anti-BRE1-A (RNF20) Antibody, clone 8A4.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: RNF20, BRE1, BRE1A UniProt Number: Q5VTR2
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MABE948
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE948
      DescriptionAnti-BRE1-A (RNF20) Antibody, clone 8A4.2
      Alternate Names
      • E3 ubiquitin-protein ligase BRE1A
      • BRE1 E3 ubiquitin ligase homolog
      • BRE1-A
      • hBRE1
      • Homolog of S. cerevisiae BRE1
      • RING finger protein 20
      Background InformationE3 ubiquitin-protein ligase BRE1A (UniProt Q5VTR2; also known as BRE1 E3 ubiquitin ligase homolog, BRE1-A, hBRE1, Homolog of S. cerevisiae BRE1, RING finger protein 20) is encoded by the RNF20 (also known as BRE1, BRE1A) gene (Gene ID 56254) in human. BRE1A (RNF20) is a RING finger domain-containing protein originally identified as the E3 uqibiquitin ligase that catalyzes histone H2B Lys120 monoubiquitination in yeast. The histone chaperone FACT is reported to mediate RNF20 recruitment for chromatin remodeling to initiate homologous recombination repair (HRR). RNF20 is also shown to negatively regulate hepatic fatty acid metabolism by targeting SREBP1c for ubiquitination and subsequent proteasomal degradation. In addition, RNF20 is shown to mediated the polyubiquitination of ErbB3-binding protein 1 (Ebp1) and Transcription factor activator protein 2α (AP-2α). AP-2α functions as a negative regulator of adipogenesis by suppressing the transcription of CCAAT/enhancer binding protein (C/EBPα). Overexpression of RNF20 in 3T3-L1 cells results in a down-regulation of endogenous AP-2α and a concomitant transcriptional upregulation of C/EBPα.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected BRE1-A (RNF20) in 10 µg of HEK293 cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the third coiled-coil region of human BRE1-A (RNF20).
      EpitopeThird coiled-coil region.
      Clone8A4.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF20
      • BRE1
      • BRE1A
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~130 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected BRE1-A (RNF20) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE948 04055977311808

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies