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GF35L Anti-BDNF Mouse mAb (35928.11)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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GF35L-100UG
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      Description
      OverviewRecognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
      Catalogue NumberGF35L
      Brand Family Calbiochem®
      SynonymsAnti-Brain Derived Neurotrophic Factor
      References
      ReferencesRiccio, A., et al. 1997. Science 277 1097.
      Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
      Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
      Gotz, R., et al. 1994. Nature 372, 266.
      Snider, W.D. 1994. Cell 77, 627.
      Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, 5% trehalose.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Neutralization Studies
      Application NotesELISA (0.5-1 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Immunohistochemistry (5-15 µg/ml)
      Neutralization Studies (see comments)
      Application CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
      ImmunogenHuman
      Clone35928.11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      GF35L-100UG 04055977220124