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ABN910-25UG Anti-Aquaporin 4

Anti-Aquaporin 4, Cat. No. ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Aquaporin 4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Aqp4 UniProt Number: P47863
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ABN910-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN910-25UG
      DescriptionAnti-Aquaporin 4
      Alternate Names
      • AQP-4
      • Mercurial-insensitive water channel
      • MIWC
      Background InformationAquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel, MIWC) is encoded by the Aqp4 gene (Gene ID: 25293) in rat. Aquaporin-4 is a multi-pass membrane homotetrameric protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Aquaporin 4, Cat. No. ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
      Biological Information
      ImmunogenGST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Aqp4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse cerebral cortex tissue.

      Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution of this antibody detected Aquaporin 4 in mouse cerebral cortex tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN910-25UG 04054839557866