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NE1004
Sigma-AldrichAnti-Apolipoprotein E Mouse mAb (E6D7)
Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.
More>>Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections. Less<<
Anti-Apolipoprotein E Mouse mAb (E6D7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
Catalogue Number
NE1004
Brand Family
Calbiochem®
Synonyms
Anti-ApoE
Application Data
Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence. Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB. Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
References
References
Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
ELISA (see comments) Immunoblotting (1:100-1:1000) Immunoprecipitation (see comments) Frozen Sections (1:100-1:10,000) Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Application Comments
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
Immunogen
Human
Clone
E6D7
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-Apolipoprotein E Mouse mAb (E6D7) Certificates of Analysis
Title
Lot Number
NE1004
References
Reference overview
Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-October-2010 JSW
Synonyms
Anti-ApoE
Application
ELISA (see comments) Immunoblotting (1:100-1:1000) Immunoprecipitation (see comments) Frozen Sections (1:100-1:10,000) Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Application Data
Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence. Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB. Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
Description
Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
Background
Apolipoprotein E (Apo E) is a polymorphic, multifunctional protein synthesized by liver, kidney, brain, and other cell types. It has been reported that the genetic inheritance of apolipoprotein isoform E4 is a significant risk factor for late onset Alzheimer's Disease (AD).
Host
Mouse
Immunogen species
Human
Immunogen
a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
Clone
E6D7
Isotype
IgG₁
Species
human, not mouse, not rabbit
Positive control
Brain tissue, serum
Form
Liquid
Formulation
Culture supernatant diluted in PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
None
Comments
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
