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MABN1205 Anti-Agrin Antibody, clone 1H10.1

Anti-Agrin, clone 1H10.1, Cat. No. MABN1205, is a highly specific mouse monoclonal antibody that targets Agrin and has been tested for use in Western Blotting.

Anti-Agrin Antibody, clone 1H10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: AGRN, AGRIN UniProt Number: O00468
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MABN1205
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1205
      DescriptionAnti-Agrin Antibody, clone 1H10.1
      Alternate Names
      • Agrin Isoform 1
      Background InformationAgrin (UniProt: O00468) is encoded by the AGRN (also known as AGRIN) gene (Gene ID: 375790) in human. Seven different isoforms of agrin have been described that are produced by alternative splicing. This antibody (clone 1H10.1) is raised against isoform 1 and is also known as secreted agrin. This isoform is a heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. It is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of acetylcholine receptors (AChR) in the postsynaptic membrane. Calcium ions reported to be essential for maximal AChR clustering. In the central nervous system it inhibits the alpha(3)-subtype of Na+/K+-ATPase and evokes depolarization at CNS synapses. This isoform of agrin forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the N-terminal (NtA) domain. At synaptic junctions, agrin is cleaved at two conserved sites, alpha and beta, by neurotrypsin. Cleavage at the alpha-site produces the agrin N-terminal 110-kDa subunit and the agrin C-terminal 110-kDa subunit. Further cleavage of agrin C-terminal 110-kDa subunit at the beta site produces the C-terminal fragments, agrin C-terminal 90 kDa fragment and agrin C-terminal 22 kDa fragment. The N-terminal (110 kDa) subunit is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. The C-terminal (22 kDa) fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. Excessive cleavage at the beta-site releases large amounts of the 22 kDa fragment that leads to destabilization at the neuromuscular junction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Agrin, clone 1H10.1, Cat. No. MABN1205, is a highly specific mouse monoclonal antibody that targets Agrin and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Agrin in 10 µg of mouse brain tissue lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 99 amino acids from the C-terminal region of human Agrin isoform 1.
      Clone1H10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1H10.1 detects Agrin in human, mouse, and rat cells. It targets an epitope within 99 amino acids from the C-terminal end.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AGRN
      • AGRIN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 217.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Agrin in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1205 04054839324314