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MABN704 Anti-ASS1 Antibody, clone 2C10

Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.

Anti-ASS1 Antibody, clone 2C10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: ASS, CTLN1, ASS1 UniProt Number: P00966
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MABN704
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, MkWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN704
      DescriptionAnti-ASS1 Antibody, clone 2C10
      Alternate Names
      • Argininosuccinate synthase
      • Citrulline--aspartate ligase
      • ASS1
      Background InformationArgininosuccinate synthase (ASS1) or alternatively known by the older name of Citrulline-Aspartate ligase is encoded by the ASS1/ASS gene in humans and is the enzyme that is responsible for the third step in the urea cycle critical to the metabolism of nitrogen. ASS1 catalyzes the synthesis of argininosuccinate from citrulline and aspartate. Argininosuccinate is the immediate precursor of arginine and ASS1 was first identified as the rate limiting step in the liver for this reaction. ASS1 is now recognized to be expressed ubiquitously in mammalian tissues. ASS1 also performs the rate limiting step for NO production in the citrulline-NO cycle as well. Regulation of ASS1 expression can vary depending upon the tissue examined. In the liver, where arginine is hydrolyzed to form urea and ornithine, ASS1 is highly expressed, and various hormones such as glucocorticoids, glucagon, insulin and even high dietary levels of specific amino acids like glutamine regulate expression of ASS1 during development and adult life. In other tissues and in particular in nitric oxide (NO) producing cells, ASS1 is expressed at a low level but induced by proinflammatory cytokines. Interestingly ASS1 expression is also increased in Alzheimer’s disease in both neurons and glia, which also is correlated with iNOS expression and suggests a link between increased NO production and iNOS expression observed in Alzheimer brains. EMD-Millipore’s Anti-ASS1 monoclonal antibody has been tested in western blot on recombinant protein and cell lysates from A431, Raji, MOLT4, Jurkat, A549, NIH3T3, PC-12, and COS7 and in paraffin embedded immunohistochemistry on cervical cancer tissue sections. The antibody has also been tested in direct ELISA on recombinant antigen.
      References
      Product Information
      FormatPurified
      Control
      • A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates
      PresentationPurified mouse monoclonal in PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ASS1 in human cervical cancer and colon cancer tissues.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human ASS1 expressed in E. Coli.
      Clone2C10
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ASS
      • CTLN1
      • ASS1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~47 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN704 04053252918070

      Documentation

      Anti-ASS1 Antibody, clone 2C10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ASS1 Antibody, clone 2C10 Certificates of Analysis

      TitleLot Number
      Anti-ASS1, clone 2C10 - VP1405163 VP1405163
      Anti-ASS1, clone 2C10 - QVP1305301 QVP1305301
      Anti-ASS1, clone 2C10 - VP2409057 VP2409057
      Anti-ASS1, clone 2C10 -VP1412164 VP1412164
      Anti-ASS1, clone 2C10 -VP1507211 VP1507211
      Anti-ASS1, clone 2C10 -VP1508201 VP1508201
      Anti-ASS1, clone 2C10 -VP1512153 VP1512153
      Anti-ASS1, clone 2C10 -VP1603025 VP1603025
      Anti-ASS1, clone 2C10 -VP1606071 VP1606071
      Anti-ASS1, clone 2C10 Monoclonal Antibody VP1805310
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