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07-302 Anti-ASK1 Antibody

07-302
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-302
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ASK1 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect ASK1 using this Anti-ASK1 Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acids 1276-1291(EEKDQEIKHLKLKSQP-C) of human ASK1
      HostRabbit
      SpecificityASK 1
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
      Gene Symbol
      • MAP3K5
      • MEKK5
      • ASK1
      • ASK-1
      • MAPKKK5
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99683 # Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
      COFACTOR: Magnesium.
      SIZE: 1374 amino acids; 154537 Da
      SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP.
      TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas.
      SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight~155kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from human SW1353 cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-302 04053252671135

      Documentation

      Anti-ASK1 Antibody Certificates of Analysis

      TitleLot Number
      Anti-ASK1 - 22224 22224

      References

      Reference overviewPub Med ID
      STAT2 deficiency and susceptibility to viral illness in humans.
      Hambleton, S; Goodbourn, S; Young, DF; Dickinson, P; Mohamad, SM; Valappil, M; McGovern, N; Cant, AJ; Hackett, SJ; Ghazal, P; Morgan, NV; Randall, RE
      Proceedings of the National Academy of Sciences of the United States of America  110  3053-8  2013

      Show Abstract
      23391734 23391734
      Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1.
      Matsuzawa, A and Ichijo, H
      J. Biochem., 130: 1-8 (2001)  2001

      Show Abstract
      11432772 11432772
      Laminar flow inhibits TNF-induced ASK1 activation by preventing dissociation of ASK1 from its inhibitor 14-3-3.
      Liu, Y, et al.
      J. Clin. Invest., 107: 917-23 (2001)  2001

      Show Abstract
      11285311 11285311

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies