Millipore Sigma Vibrant Logo

MABN381 Anti-APP-C99 Antibody, clone mC99(1-7)

View Products on Sigmaaldrich.com
MABN381
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Fulfillment and Delivery Delayed
Fulfillment and Delivery Delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICC, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN381
      DescriptionAnti-APP-C99 Antibody, clone mC99(1-7)
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD). UP on intracerebroventricular injection of this antibody, A beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN380, Anti-APP-C99, clone mC99(70-80) was raised against a slightly different region of the same APP-C99 protein.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgGκ ascites
      Quality LevelMQ100
      Applications
      ApplicationAnti-APP-C99 Antibody, clone mC99(1-7) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

      Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
      (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

      Immunofluoresence Anlaysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
      Biological Information
      ImmunogenRecombinant protein corresponding to Human APP-C99.
      ClonemC99(1-7)
      HostMouse
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DAPT treated HEK293 cell lysate.

      Western Blotting Anlaysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN381 04053252918827

      Documentation

      Anti-APP-C99 Antibody, clone mC99(1-7) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-APP-C99 Antibody, clone mC99(1-7) Certificates of Analysis

      TitleLot Number
      Anti-APP-C99, clone mC99 (1-7) Monoclonal Antibody 2886935
      Anti-APP-C99, clone mC99(1-7) - 2952581 2952581
      Anti-APP-C99, clone mC99(1-7) - 3187490 3187490
      Anti-APP-C99, clone mC99(1-7) - 3888730 3888730
      Anti-APP-C99, clone mC99(1-7) - 4195426 4195426
      Anti-APP-C99, clone mC99(1-7) - Q2226884 Q2226884
      Anti-APP-C99, clone mC99(1-7) -2843852 2843852
      Anti-APP-C99, clone mC99(1-7) Monoclonal Antibody 3108549

      References

      Reference overviewPub Med ID
      Selective neutralization of APP-C99 with monoclonal antibodies reduces the production of Alzheimer's Aβ peptides.
      Houacine, Jemila, et al.
      Neurobiol. Aging, 33: 2704-14 (2012)  2012

      Show Abstract
      22317957 22317957

      Newsletters / Publications

      Title
      Research Focus - Volume 2, 2013

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies