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DR1090 Anti-AHR Mouse mAb (3B12)

Anti-AHR Mouse mAb (3B12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aryl Hydrocarbon Receptor

DR1090
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~100 kDa AHR protein in COLO 320 HSR and HeLa cells.
      Catalogue NumberDR1090
      Brand Family Calbiochem®
      SynonymsAnti-Aryl Hydrocarbon Receptor
      Application Data
      Detection of human AHR by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human AHR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesSchults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T
      Positive controlCOLO 320 HSR cells, HeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 721-821 human AHR, expressed as a GST fusion protein
      Clone3B12
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1090 0

      Documentation

      References

      Reference overview
      Schults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Aryl Hydrocarbon Receptor
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human AHR by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human AHR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa AHR protein.
      BackgroundThis protein is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 721-821 human AHR, expressed as a GST fusion protein
      Clone3B12
      IsotypeIgG₁
      Specieshuman
      Positive controlCOLO 320 HSR cells, HeLa cells
      Negative control293T
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesSchults, M,A., et al. 2010. J. Biol. Chem. 285, 14558.