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PC528 Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb

Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Kuz

PC528
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.



      Please refer to our complete listing of ADAM10 antibodies for possible alternatives.






      Recognizes the ~85 kDa ADAM10 precursor, a faint 60 kDa band corresponding to processed mature protein, and a 45 kDa degradation product in Jurkat cells.
    • Antibody Target Gene Symbol: ADAM10
    • Target Synonym: 1700031C13Rik, AD10, ALPHA SECRETASE, CD156c, DISINTEGRIN-METALLOPROTEASE, HsT18717, Kuz, KUZBANIAN, MADM
    • Entrez Gene Name: ADAM metallopeptidase domain 10
    • Hu Entrez ID: 102 (Related Antibodies: 422751)
    • Mu Entrez ID: 11487
    • Rat Entrez ID: 29650
      		•
      Catalogue NumberPC528
      Brand Family Calbiochem®
      SynonymsAnti-Kuz
      Application Data
      Detection of human ADAM10 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADAM10 (Ab-1) Rabbit pAb (Cat. No. PC528) (1:1000) pre-incubated without (lane 1) or with (lane 2) blocking peptide. Detection: chemiluminescence
      References
      ReferencesQi, H., et al. 1999. Science 283, 91.
      Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
      Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
      Howard, L., et al. 1996. Biochem. J. 317, 45.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlJurkat cell lysate
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500-1:2000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (PQRQRPRESYQMGHMRR) corresponding to amino acids 732-748 of human ADAM10
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC528 0

      Documentation

      Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC528

      References

      Reference overview
      Qi, H., et al. 1999. Science 283, 91.
      Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
      Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
      Howard, L., et al. 1996. Biochem. J. 317, 45.

      Citations

      Title
    • Daniel W. Lambert, et al. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the SARS-CoV receptor, angiotensin-converting enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2007 RFH
      SynonymsAnti-Kuz
      ApplicationImmunoblotting (1:500-1:2000)
      Application Data
      Detection of human ADAM10 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADAM10 (Ab-1) Rabbit pAb (Cat. No. PC528) (1:1000) pre-incubated without (lane 1) or with (lane 2) blocking peptide. Detection: chemiluminescence
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~85 kDa ADAM10 protein.
      BackgroundProinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including T-cell lymphoma. TNF-α and its ligand delta are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptors. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM, was recently identified to cleave TNF-α, notch, and it's ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10, an ~85 kDa protein, is expressed in a variety of human and bovine tissues.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (PQRQRPRESYQMGHMRR) corresponding to amino acids 732-748 of human ADAM10
      IsotypeIgG
      Specieshuman
      Positive controlJurkat cell lysate
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freze (-20°C).
      Toxicity Standard Handling
      ReferencesQi, H., et al. 1999. Science 283, 91.
      Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271.
      Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588.
      Howard, L., et al. 1996. Biochem. J. 317, 45.
      Citation
    • Daniel W. Lambert, et al. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the SARS-CoV receptor, angiotensin-converting enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
      		•