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MABC697 Anti-ACLY, clone 5F8D11 Antibody

This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.

Anti-ACLY, clone 5F8D11 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ACLY UniProt Number: P53396
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MABC697
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, MkWB, IHC, IF, FC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC697
      DescriptionAnti-ACLY, clone 5F8D11 Antibody
      Alternate Names
      • ATP-citrate synthase
      • ATP-citrate (pro-S-)-lyase
      • ACL
      • Citrate cleavage enzyme
      Background InformationATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      • Flow Cytometry
      • ELISA
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue.

      Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells.

      ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human ACLY expressed in E. Coli
      Clone5F8D11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ACLY
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC697 04053252955259

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies