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AM63 Anti-A20 Mouse mAb (59A426)

Anti-A20 mouse monoclonal antibody, clone 59A426, recognizes ~70 kDa A20 protein in Daudi cells. It is validated for use in immunoblotting and immunoprecipitation.

Anti-A20 Mouse mAb (59A426) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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AM63
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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AM63-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~70 kDa A20 protein in Daudi cells.
      Catalogue NumberAM63
      Brand Family Calbiochem®
      Application Data
      Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHeyninck, K., et al. J. Cell Biol 145, 1471.
      May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
      Tewari, M., et al. 1995. J. Immunol. 154, 1699.
      Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Positive controlDaudi cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:500-1:2000)
      Immunoprecipitation (2 µg/mg)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length recombinant human A20 protein
      ImmunogenHuman
      Clone59A426
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM63-100UG 04055977228021

      Documentation

      Anti-A20 Mouse mAb (59A426) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-A20 Mouse mAb (59A426) Certificates of Analysis

      TitleLot Number
      AM63

      References

      Reference overview
      Heyninck, K., et al. J. Cell Biol 145, 1471.
      May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
      Tewari, M., et al. 1995. J. Immunol. 154, 1699.
      Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.

      Brochure

      Title
      Antibody Sourcebookﾙ Sixth Edition
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      ApplicationImmunoblotting (1:500-1:2000)
      Immunoprecipitation (2 µg/mg)
      Application Data
      Detection of human A20 by immunoblotting. Sample: Whole cell lysate (10 µg) from Daudi cells. Primary antibody: Anti-A20 Mouse mAb (59A426) (Cat. No. AM63) (3 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~70 kDa A20 protein.
      BackgroundNF-κB (nuclear factor κB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-κB gene expression by interferring with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IκB kinase pathway. A20, an 70 kDa protein, is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.
      HostMouse
      Immunogen speciesHuman
      Immunogenfull-length recombinant human A20 protein
      Clone59A426
      IsotypeIgG₁
      Specieshuman
      Positive controlDaudi cells
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHeyninck, K., et al. J. Cell Biol 145, 1471.
      May, M.J. et al. 1997. Seminars in Cancer Biology. 2, 63.
      Tewari, M., et al. 1995. J. Immunol. 154, 1699.
      Opipari, A.W., et al. 1990. J. Biol. Chem. 265, 14705.