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ABE1439 Anti-60S RPL5

Anti-60S RPL5, Cat. No. ABE1439, is a highly specific rabbit polyclonal antibody that targets 60S ribosomal protein L5 and has been tested in Immunocytochemistry and Western Blotting.

Anti-60S RPL5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: RPL5, DBA6, MSTP030 UniProt Number: P46777
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ABE1439
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1439
      DescriptionAnti-60S RPL5
      Alternate Names
      • 60S ribosomal protein L5
      • RPL5
      Background Information60S ribosomal protein L5 (UniProt P46777; also known as RPL5) is encoded by the RPL5 (also known as DBA6, MSTP030) gene (Gene ID 6125) in human. Ribosomal proteins (RPs) are normally involved in the assembly of the translational machinery-ribosomes essential for protein production. RPL5, RPL11 and the 5S rRNA comprise the 5S ribonucleoprotein particle (RNP), an essential subcomplex of the large ribosomal subunit. RPL5 binds the 5S rRNA, the 5S rRNA/RPL5 complex then localizes to the nucleolus, where it binds RPL11 and is integrated into the ribosome. In response to ribosomal stress (RS) or nucleolar stress, the L5/L11/5S pre-ribosomal complex is redirected to inhibit MDM2 E3 ligase activity, thereby promoting p53 stabilization and p53-dependent cell cycle arrest. RPL5 and RPL11 can also bind TAp73 N-terminal TA domain in an RS-independent manner and thereby prevent MDM2 association with TAp73 target gene promoters, resulting in enhanced TAp73 transcriptional activity and TAp73-dependent apoptosis induction and cell cycle arrest.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-60S RPL5, Cat. No. ABE1439, is a highly specific rabbit polyclonal antibody that targets 60S ribosomal protein L5 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected 60S RPL5 in human (A431, HeLa, HepG2) and mouse (NIH/3T3) cells.

      Western Blotting Analysis: 1 µg/mL from a representative lot detected 60S RPL5 in 10 µg of human (A431 epidermoid carcinoma & HUVEC) and rat (L6 myoblast) cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the C-terminal half of human 60S RPL5.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets a highly conserved sequence within the C-terminal half of 60S RPL5 from multiple species.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Horse, Porcine, Rabbit, and Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPL5
      • DBA6
      • MSTP030
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~36 kDa observed. 34.23/34.27/34.33 kDa (human/mouse/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected 60S RPL5 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1439 04054839080807