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PF036 Annexin V-Biotin Apoptosis Detection Kit

Annexin V-Biotin Apoptosis Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PF036
  
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      Overview

      Key Spec Table

      Detection Methods
      Fluorescence
      Description
      OverviewNovel RAPID™ protocol and easy sample preparation. Optimized for flow cytometry and fluorescence microscopy applications. Useful for detection of membrane changes during apoptosis. Also useful in conjugation with GFP or other fluorophores.
      User provides appropriately labeled streptavidin.
      Catalogue NumberPF036
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Streptavidin or anti-biotin conjugated to a fluorescent molecule such as fluorescein isothiocyanate (FITC), cyanine 3 (Cy3), Alexa™488, or phycoerythrin (PE)
      • PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4)
      • 2-20 µl, 20-200 µml, and 200-1000 µl precision pipettors with disposable tips
      • Microcentrifuge tubes
      • Adjustable speed microcentrifuge
      • Glass microscope slides (for microscope analysis only)
      • Glass coverslips (for microscope analysis only)
      • dH2O
      • Ice
      • Fluorescence microscope or flow cytometer
      References
      ReferencesDarzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
      Frey, T. 1997. Cytometry 28, 253.
      Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
      White, E. 1996. Genes and Devel. 10, 1.
      Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
      Koopman, G., et al. 1994. Blood 84, 1415.
      Wyllie, A.H. 1993. Br. J. Cancer 67, 205.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
      Schmid, I., et al. 1992. Cytometry 13, 204.
      Kerr, J.F.R., et al. 1972. Cancer 26, 239.
      Product Information
      Detection methodFluorescence
      DeclarationSold under license of U.S. Patent 5,834,196.
      Form100 Tests
      FormatFlow cytometry or fluorescence microscopy
      Kit containsAnnexin V-biotin, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
      Positive controlApoptotic Jurkat cells (dexamethasone or anti-Fas treated)
      Applications
      Key Applications Flow Cytometry
      Immunofluorescence
      Biological Information
      Assay time15 - 30 min
      Sample TypeIntact cells
      Species Reactivity
      • A Broad Range Of Species
      Product Usage Statements
      Intended useThe Calbiochem® Annexin V-Biotin Apoptosis Detection Kit is a non-isotopic system for the identification of cell membrane alterations that accompany programmed cell death. Detection may be performed by flow cytometry or by fluorescence microscopy using various fluorescent streptavidin or anti-biotin conjugates.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsAnnexin V-BIOTIN kit components are shipped on gel ice pack. Upon receipt, store kit at 4°C. To avoid reagent loss in tube caps, briefly pulse spin all small tubes before removing caps. See Precautions and Recommendations.
      Do not freeze Yes
      Supplemental Information
      Kit containsAnnexin V-biotin, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      PF036 0