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AAMT009 Aconitase Activity Assay Kit

Aconitase Activity Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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AAMT009
  
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      Overview

      Replacement Information
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      Overview

      This product has been discontinued.



      Product Description

      The active form of the enzyme is inhibited by citrate analogs, and fluoracetate. Other inhibitors include oxidative stress agents such as peroxynitrite, hydrogen peroxide and superoxide, which inactivate the enzyme by changing the [4Fe-4S] to a [3Fe-4S] cluster. Therefore, aconitase is considered a good marker of mitochondrial and cellular oxidative stress. This inhibition of mitochondrial aconitase can lead to a decreased energy production, whereas in cytosolic aconitase it triggers binding of the enzyme to mRNA iron response elements resulting in increased expression of iron uptake proteins and decreased transcription of iron sequestering protein.

      A hydroxyl scavenging solution (Aconitase preservation solution) is supplied with this kit to maintain aconitase activity during sample preparation. An inactivated [3Fe-4FS] aconitase may be activated in vitro by the addition of iron and cysteine.

      Citrate  ←→  cis-aconitate   ←→  isocitrate

      (↑ Abs. 240 nm)


      The Aconitase Activity Assay Kit measures the activity of aconitase in a sample by following the conversion of isocitrate to cis-aconitate as indicated by an increase in absorbance at UV 240 nm with an extinction coefficient of 2.2 OD/mM per well.

      Note: Mitochondrial and cytoplasmic aconitase activities are indistinguishable. Therefore, to measure the mitochondrial activity only, first isolate mitochondria, but to measure for both activities, separate the cells into cytoplasmic and mitochondrial fractions (see TB552 for details).

      The entire assay can be completed within 2 hours. The intra-assay variation and inter-assay variation with this assay are each less than 10% and 15% respectively.

      Panel Components

      50 ml 	Buffer (Tube 1), (72575-50ML)
20 ml	Aconitase preservation solution, (72576-20ML)
800 µl  	25X Isocitrate, (72577-800UL)
200 µl	100X Manganese, (72578-200UL)
1 ea	UV Microplate, (72579-1EA)
1 ml	Detergent, (72580-1ML)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
      Storage: Store all components at 2 to 8°C. Do not freeze.

      Note: The 96-well microplate has a monoclonal antibody pre-bound to the wells. This plate can be separated into twelve 8 well strips and thus can be used for up to 12 individual experiments.
      Catalogue NumberAAMT009
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AAMT009 0