Millipore Sigma Vibrant Logo

101597 AFB-Fluor phenol-free

Staining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)

AFB-Fluor phenol-free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
101597
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1015970001
Retrieving availability...
—
Fulfillment and Delivery Delayed Fulfillment and Delivery Delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Fibre case 1 set
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      Catalogue Number101597
      DescriptionAFB-Fluor phenol-free
      OverviewThe decisive feature of the AFB-Fluor phenol-free for detection of aldehyde and mucosubstances, is the presence of fluorescence dyes in the staining solution. This enables the swift and secure identification of the acid-fast bacteria under the fluorescence microscope. As it has become an established procedure alongside Ziehl-Neelson staining (AFB-Color modified), we provide three ready-to-use solutions in our AFB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Cat.no. 100497) (ZN) is possible. The AFB-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.
      The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3212 90 90
      Quality LevelMQ400
      Applications
      ApplicationStaining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H225: Highly flammable liquid and vapour.
      H290: May be corrosive to metals.
      H319: Causes serious eye irritation.
      H336: May cause drowsiness or dizziness.
      H411: Toxic to aquatic life with long lasting effects.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P234: Keep only in original packaging.
      P240: Ground and bond container and receiving equipment.
      P273: Avoid release to the environment.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P403 + P235: Store in a well-ventilated place. Keep cool.
      P501: Dispose of contents/ container to an approved waste disposal plant.
      Signal WordDanger
      Storage class3 Flammable liquids
      Safety Information
      Categories of dangerflammable
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3316 Entzündbarer flüssiger Stoff, ätzend, n.a.g.(SALZSAEURE UND ENTH. ISOPROPANOL), 9, II
      Declaration (transport by air) IATA-DGRUN 3316 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ISOPROPANOL), 9, II
      Declaration (transport by sea) IMDG-CodeUN 3316 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ISOPROPANOL), 9, II
      Supplemental Information
      Specifications
      Suitability for microscopypasses test
      Mycobacteriared-orange/yellow-green
      Backgrounddark
      Global Trade Item Number
      Catalogue Number GTIN
      1015970001 04022536977386