2015 Merck Asia Forum: "New Flow, New Thinking"

March 9-10, Singapore

• Speaker list and biography

  Kathleen McGrath, Ph.D. Title / Designation: Research Assistant Professor, Department of Pediatrics, Hematology and Oncology (SMD) and the Center for Pediatric Biomedical Research Institution:University of Rochester Medical Center, School of Medicine and Dentistry Instrument in Laboratory: ImageStreamX Topic of the presentation: "Delineating Hematopoietic Development by Imaging Flow Cytometry" Seminar Abstract: During adult hematopoiesis, pluripotent hematopoietic stem cells (HSC) in the bone marrow differentiate into specific progenitors that then progress through maturation of precursors to produce the diverse array of blood cells found in our bodies. However, it is clear that this hematopoiesis in the embryo is very different. Hematopoiesis in the mammalian embryo is not at steady state, but dramatically expands in cell number and in lineage complexity during gestation. Additionally, hematopoietic function is required before HSC are formed in the embryo. Embryos solve this dilemma by creating additional sources of hematopoietic cells that arise pre-HSC. These sequential waves of hematopoiesis display differences in self-renewal, potential and blood cell function that are relevant to our understanding of hematopoietic regulation, as well as pediatric diseases and embryonic stem cell maturation. While understanding embryonic hematopoiesis is important, it presents several challenges including; rarity of the cells, subtle differences between overlapping hematopoietic waves, and maturation defined by flow cytometric and morphologic criteria. Our studies have made extensive use of imaging flow cytometry (ImageSteamX). The advantage of imaging flow cytometry (IFC) is that large numbers of cells can be analyzed with each pixel of the cells image being independently compensated allowing accurate quantitation. Utilizing this technique, we have been able integrate morphological characteristics, such as size and shape of the cell and nucleus, with flow cytometric parameters, such as immunophenotype, ploidy, cell cycle status, and mitochondrial content. Furthermore, IFC has the unique capability to quantitating not only the fluorescent signal strength, but also the morphology of that signal. This has allowed more powerful discrimination of apoptosis, DNA damage (gamma H2A spots) and cell signaling (nuclear localization of phosphoSTAT proteins). It also allows discrimination of cells with legitimate staining from confounding debris displaying artifactual staining that is particularly critical for rare populations like megakaryocytes. These data have contributed to the model of successive waves of primitive and definitive hematopoiesis that arise pre-HSC in the yolk sac and are recapitulated in developing embryonic stem cell systems. Speaker Biography: Kathleen E. McGrath received her BA in Biology at Reed College in Portland, Oregon and PhD in Molecular Biology at the University of Rochester, New York. Since 2001, she has been a Research Assistant Professor at the University of Rochester Medical School in the Center for Pediatric Biomedical Research. Her research focuses on the emergence of the hematopoietic system in the murine embryo as well as the recovery of hematopoiesis after injury. Imaging flow cytometry has been an important tool in many aspects of these studies, and she has participated as an instructor in imaging cytometry at the Annual Course in Flow Cytometry held at Bowdoin College.

  Kevin SW TAN, BSc (Hons), Ph.D, CBiol, MSB Title / Designation: Associate Professor, Department of Microbiology Institution: National University of Singapore - Department of Microbiology, Laboratory of Molecular and Cellular Parasitology Instrument in Laboratory: ImageStreamX-MKII Topic of the presentation: "High-Content Phenotypic Assays for Accelerated Antimalarial Drug Discovery" Seminar Abstract: Malaria is an infectious disease of global importance, claiming hundreds of thousands of lives annually. A major hurdle to malaria eradication is the problem of drug resistance. Drugs with new mechanisms of action are urgently required. Our laboratory has identified a novel drug-induced death pathway that involves the digestive vacuole (DV) of the malaria parasite. Using a high content imaging system, we have identified new and old drugs that disrupt the parasite DV, resulting in parasite death. Exploiting our knowledge on death pathways, and combining this with cutting-edge high content cytometric approaches, has enormous potential in accelerating drug discovery. Speaker Biography: Kevin Shyong-Wei TAN is Associate Professor at the Department of Microbiology, National University of Singapore. He is also Founding Director of BioLynx Technologies, a local biotech spinout company, focusing on the commercialization of molecular probes as research tools. His curiosity for parasites originated from his graduate student days at NUS and blossomed during his postdoctoral stint at The Rockefeller University, New York City. He is relieved to be awarded tenure in 2011, and can now spend more time on social issues, such as public science education. Kevin's research focuses on understanding how parasites commit suicide and exploiting such knowledge to trigger death mechanisms as an anti-parasite strategy. He is also interested in the problem of drug resistance and his team has recently come up with new ways to find drugs that overcome resistance. He hopes that the research from his team would accelerate the finding of new cures for parasitic diseases. Main Research Interests: The protozoan parasites Plasmodium falciparum and Blastocystis: host-pathogen interactions Plasmodium falciparum is the most virulent of the human malaria parasites and causes the most devastating parasitic disease resulting in about 1-3 million deaths annually. Our laboratory, together with collaborators at the Department of Chemistry, is focused on the development of fluorescent-labeled drugs as probes for drug screening, diagnostics and understanding parasite biology. Our laboratory has contributed extensively to the current understanding of Blastocystis biology. Our research on this protist focuses on host-pathogen interactions, which aims to clarify its controversial role in intestinal disease. We have shown that the parasite exhibits IgA protease activity, induces apoptosis in host cells, and compromises barrier function in intestinal epithelial cells. Current work involves characterization of Blastocystis virulence factors, pathogenesis and host response by molecular and cell biology approaches. Protozoan programmed cell death We are also studying the phenomenon of programmed cell death (PCD) in protozoan parasites using Blastocystis and P. falciparum as model organisms. Our work on both parasites has shown that a variety of external stimuli can trigger PCD with features similar to those reported for mammalian cells, including links between cysteine protease activation/ mitochondrial factors and DNA fragmentation. We are currently studying the molecules and organelles that are involved in the induction and regulation of PCD in P. falciparum and Blastocystis, with particular focus on protease-dependent and –independent pathways. The identification of novel activators of protozoan PCD provide exciting possibilities for anti-parasite strategies.

  Sook-Kyoung Heo, Ph. D. Title / Designation: Research professor, Biomedical Research Center Institution: University of Ulsan, Ulsan, Republic of Korea Instrument in Laboratory: FlowSight with Quantitative Imaging (QI) Topic of the presentation: "Rosmarinic acid potentiates ATRA-induced macrophage differentiation in acute promyelocytic leukemia NB4 Cells" Seminar Abstract: Rosmarinic acid (RA, an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid) has a number of biological activities, but little is known about anti-leukemic activities of RA combined with all-trans retinoic acid (ATRA) against acute promyelocytic leukemia (APL) cells. We examined the differentiation marker, CD11b, in bone marrow cells (BMC) of an APL patient, in NB4 cells (APL cell line), and in normal BMC and peripheral blood mononuclear cells (PBMC) of healthy subjects by flow cytometric analysis. ATRA/RA induced expression of CD11b in the BMC of the APL patient and in NB4 cells, but not in normal BMC or PBMC. Therefore, we realized that RA potentiated ATRA-induced macrophage differentiation in APL cells. Further characterization of the induced macrophages showed that they exhibited morphological changes and were able to phagocytose and generate reactive oxygen species. These macrophages also had typical expression of C-C chemokine receptor type 1 (CCR1), CCR2, and intercellular adhesion molecule-1 (ICAM-1). Moreover, the expression of CD11b+ and CD14+ cells depended on ERK-NF-κB axis activation. Together, these results indicate that RA potentiates ATRA-induced macrophage differentiation in APL cells. Thus, RA may play an important role as an appurtenant differentiation agent for functional macrophage differentiation in APL. Additionally, the differentiated macrophages might have a normal life span and, they could die. These data indicate that co-treatment with RA and ATRA has potential as an anti-leukemic therapy in APL. Keywords: Rosmarinic acid, All-trans retinoic acid, Macrophage differentiation, Acute promyelocytic leukemia, Anti-leukemic activity, Differentiation therapy. Speaker Biography: 2007. 02. Ph. D., Department of Immunology and biomedicine, University of Ulsan. 2007-2010. Research professor, Cardiovascular Medical Research Center, University of Dongguk. 2011-2013. Post-Doc, Korea Research Institute Bioscience and Biotechnology (KRIBB). 2014- Present Research professor, Biomedical Research Center, University of Ulsan.

  Brian K. McFarlin, Ph.D. Title / Designation: Associate Professor, Department of Kinesiology, Health Promotion, and Recreation Institution: University of North Texas - Applied Physiology Laboratory, USA Instrument in Laboratory: FlowSight with Quantitative Imaging (QI), autosampler, 405, 488, 642, and SSC laser Topic of the presentation: "New Insights into Chronic Disease: New Insight Gained from Image-based Flow Cytometry Analysis" Seminar Abstract: In this presentation, we will demonstrate how the FlowSight® system's increased detection sensitivity and imaging capabilities can enhance a traditional flow cytometry assay for leukocyte function. This talk will provide insight concerning how to leverage the power of image-based flow cytometry to better understand the mechanisms associated with chronic disease. Also, by leveraging the unique quantitative imaging upgrade on the FlowSight® instrument, we have been able to collect unique information beyond what is possible with traditional flow cytometry plots. Speaker Biography: Dr. Brian K. McFarlin is an Associate Professor in the Department of Kinesiology, Health Promotion and Recreation and co-director of the Applied Physiology Laboratory at the University of North Texas. Dr. McFarlin earned his PhD and completed his postdoctoral fellowship at Purdue University. In the Fall 2012 after serving on the faculty at the University of Houston for 8 years, he joined the faculty of the University of North Texas. Dr. McFarlin's research focuses on two main areas: 1) The physiological and immunologic consequences of weight gain and loss, and 2) The use of nutritional countermeasures to maximize immune health after exercise. He has published more than 75 peer-reviewed articles, and his research has been funded by numerous grants from the National Institutes of Health, private foundations, and industry sources. Dr. McFarlin is a fellow of both The Obesity Society and American College of Sports Medicine (ACSM). He also is currently serving as the Executive Director of the Texas Chapter of ACSM and as the Associate Editor-in-Chief of the International Journal of Exercise Science. Dr. McFarlin's recent efforts have focused on using image-based flow cytometry to elucidate the effects of nutrition, exercise, and extreme environments on immune system health.

  Professor Wendy Erber, MD Ph.D. Title / Designation: Professor, Chair and Head of the School of Pathology and Laboratory Medicine Institution: School of Pathology and Laboratory Medicine, The University of Western Australia Instrument in Laboratory: ImageStreamX-MKII Topic of the presentation: "Applications of imaging flow cytometry in Leukaemia diagnostics" Speaker Biography: Professor Erber graduated in Medicine with 1st class honours from the University of Sydney. She undertook her Haematology training at the Royal North Shore Hospital of Sydney and the University of Oxford (as a Rhodes Scholar). In Oxford her research led to Doctorate of Philosophy. She has held Consultant Haematologist posts in Western Australia and in Cambridge, UK. She took up her current appointment as Chair and Head of the School of Pathology and Laboratory Medicine at the University of Western Australia in 2011. Throughout her professional career Professor Erber's major interests have been the adoption of new cutting-edge technologies, translating these into diagnostic pathology practice, especially in the field of haematological malignancies. She has more than 140 publications in peer-reviewed journals and published 3 books.

  Mr. Geoffrey Osborne Title / Designation: Director of Flow Cytometry Institution: Queensland Brain Institute, The University of Queensland Brisbane, Australia Instrument in Laboratory: ImageStreamX-MKII Topic of the presentation: Integration of Flow and Image Cytometry: Applications in the Neuroscience Seminar Abstract: Flow cytometry has not traditionally been a mainstay technology in cell biology based neuroscience studies, however microscopy imaged based analysis is a widely used approach. Image cytometry draws its strength from both approaches and therefore we sought to exploit these strengths to answer questions in the areas of brain tumour and neural stem cell research that were difficult to approach with either flow cytometry or microscopy alone. The first section of this presentation will focus on our experiences in these areas. The latter part of this presentation will expand on where we see application for this technology in molecular cytometry. Recently, technological advances in the areas of probe design and reporters now make it feasible to detect by cytometry intracellular levels of mRNA and miRNA in cells. The ability to examine individual single cells at the DNA, RNA and protein level is going to be transformational in many areas of basic research. This part of the presentation aims to provide background information on these technologies and how these may integrate with existing research approaches. Speaker Biography: Mr Osborne obtained an Associate Diploma in Applied Biology in 1983, before a Bachelor of Science from the Australian National University (ANU). He began specialising in flow cytometry in 1989 at the John Curtin School of Medical Research, ANU. From 1989 to 1999 his interest lay in developing novel flow cytometry technologies in software and hardware with a particular emphasis cell sorting and high content screening applications. In 2000 he moved to the University of British Columbia, Vancouver, Canada to establish a core facility before return to ANU in 2001. He was recruited in 2004 to a joint position at The Queensland Brain Institute and the Australian Institute for Bioengineering and Nanotechnology.

  Aurore Trottet, Ph.D. Title / Designation: Senior Plankton Scientist Institution: DHI Water & Environment (S) Pte. Ltd. Instrument in Laboratory: FlowSight with Quantitative Imaging (QI) Topic of the presentation: Use of the FlowSight with Quantitative Imaging in Ballast Water Treatment System Speaker Biography: Aurore Trottet is a senior plankton researcher at DHI Singapore. She has obtained her PhD from the University of Quebec (Canada) in 2007 in Oceanography. Before joining DHI, her research was focused on anthropic impact on plankton and bacterial communities using flow cytometry. She has been working on the effect on these communities of aquaculture in Magdalen Island (Canada), flash flood in Mediterranean Lagoon (France) and black carbon in Ha Long Bay (Vietnam). At DHI, she is implied in different activities such ecological and water quality monitoring, environmental impact assessment, ecological modelling. One of her role is also to develop new testing protocols in DHI laboratory to be used for water quality analyses and Ballast Water treatment.

• Agenda

  March 9th, 2015

  TIME	TOPICS	SPEAKER(S)
  8:30 - 9:00am	Registration & Seating	ALL
  9:00 - 9:15am	Welcome & Introductions	Aman Bhattacharjee Merck Bioscience, APAC
  9:15 - 9:30am	Amnis Imaging Cytometry "ImageStream® Technology Overview"	George McBride Merck Bioscience - Emerging Asia
  9:30 - 10:30am	Opening Plenary Session: "Delineating Hematopoietic Development by Imaging Flow Cytometry"	Kathleen McGrath, PhD University of Rochester Medical Center, USA
  10:30 - 11:00am	TEA BREAK	ALL
  11:00 - 11:45am	ImageStreamX-MKII Instrument Focus "A Novel High Content Screening Approach for Drug Inducers of Digestive Vacuole Permeabilization in Plasmodium Falciparum – as a Novel Antimalarial Strategy"	Kevin Tan, PhD National University of Singapore
  11:45 - 12:00pm	Amnis "Hot-off-the-Press" from Singapore "New Data Blitzes of Imaging cytometry"	Singapore Lab Group(s) - TBD
  12:00 - 1:00pm	LUNCH	ALL
  1:00 - 1:45pm	FlowSight Instrument Focus "Applications of imaging flow cytometry in Phagocytosis & NFKB Translocation"	Sook-Kyoung Heo, PhD University of Ulsan Republic of Korea
  1:45 - 2:30pm	ImageStreamX-MKII Instrument Focus "Applications of imaging flow cytometry in leukemia diagnostics"	Professor Wendy Erber, PhD The University of Western Australia
  2:30 - 3:15pm	ImageStreamX-MKII Instrument Focus" Integration of Flow and Image Cytometry：Applications in Neuroscience"	Mr. Geoffrey Osborne, Director of Flow Cytometry University of Queensland, Brisbane, Australia
  3:15 - 3:45pm	TEA BREAK	ALL
  3:45 - 4:30pm	FlowSight Instrument Focus "New Insights into Chronic Disease: New Insight Gained from Image-based Flow Cytometry Analysis"	Brian K. McFarlin, PhD University of North Texas, USA
  4:30 - 5:00pm	Open Forum Discussion "Amnis Strategies to Advance Research"	ALL Speakers

  March 10th, 2015

  TIME	TOPICS	SPEAKER(S)
  8:30 - 9:00am	Registration & Seating	ALL
  9:00 - 9:10am	Welcome & Introductions	George McBride Merck
  9:10 - 10:00am	FlowSight Instrument Focus "Use of the FlowSight with Quantitative Imaging in Ballast Water Treatment System"	Aurore Trottet, PhD DHI Water & Environment, Pte. Ltd. Singapore
  10:00 - 10:30am	TEA BREAK	ALL
  10:30 - 11:00am	Novel Imaging Cytometry Applications "Characterization of Protein Aggregates and Silicone Oil Droplets in particle analysis of therapeutic & pharmaceutical formulations"	Sherree Friend, PhD Merck
  11:00 - 12:00am	IDEAS Software Data Workshop - Update "Image data analysis: The shortcut to separating and measuring your populations"	Sherree Friend, PhD Merck
  12:00 - 1:00pm	LUNCH	ALL
  1:00 - 3:00pm	WET WORKSHOP for New ISX-MKII Instrument (Genome Institute Laboratory) "NF-kB Translocation Demonstration" (Detect & quantitate NF-kB's translocation)	Sherree Friend, PhD Merck & ISX-MKII Lab
  1:00 - 3:00pm	WET WORKSHOP for FlowSight Instrument (Research Instruments Laboratory) "NF-kB Translocation Demonstration"(Detect & quantitate NF-kB's translocation)	Chin Chieh Loh, RI Research Instruments & Mun-Keat Chong, PhD, Merck
  3:00 - 3:30pm	TEA BREAK
  3:30 - 4:30pm	WET WORKSHOP RESULTS (FlowSight & ISX-MKII Data) "NF-kB translocation Assay" (Detect & quantitate NFkB's translocation)	Mun-Keat Chong, PhD, Merck & Sherree Friend, PhD Merck
  4:30 - 5:00pm	Amnis "Hot-off-the-Press" "New Data Blitzes of Imaging cytometry"	Group(s) - TBD
  5:00 - 5:15pm	Amnis Meeting Closing Remarks	Aman Bhattacharjee Merck Bioscience, APAC

April 14, Beijing

• Speaker list and biography

  Juan Garcia Vallejo, MD, Ph.D. Title / Designation: Senior Researcher, Department of Molecular Cell Biology & Immunology Institution: VU University Medical Center Topic of the presentation: Addressing intracellular routing of antigens in antigen-presenting cells using imaging flow cytometry Speaker Biography: Dr. Garcia-Vallejo obtained his MD from the University of Malaga (Spain) in 1999 and quickly moved into basic research in the field of Glycobiology and Immunology. His PhD dissertation on the regulation of glycosylation in endothelial cells was defended in 2005 at the Free University of Amsterdam. Dr. Garcia-Vallejo moved then to the group of Prof. van Kooyk at the VU University Medical Center, where he has worked since in the Immunobiology of C-type lectin mediated antigen uptake in dendritic cells, with a special interest in their role in the control of immune homeostasis and their potential in cancer immunotherapy.

  Orla Maguire, Ph.D. Title / Designation: Research Scientist （Flow Cytometry Specialist） Institution: Roswell Park Cancer Institute Topic of the presentation: Clinical applications of imaging flow cytometry Speaker Biography: Dr. Orla Maguire obtained her B.Sc. and Ph.D in Biomedical Science in 2004 and 2008, respectively from the University Of Ulster, Northern Ireland, UK. She started her post-Doc work in Roswell Park Cancer Institute, Buffalo, NY, USA initially with focus on Pharmacology, and then Imaging Flow Cytometry and she is now a Flow Cytometry Specialist there. Dr. Maguire is particularly interested in the clinical applications of imaging flow cytometry and has published her research on quantification of the NF-κB and NFAT signaling pathway using the ImageStream. She has also developed a novel Fluorescent In Situ Hybridization In Suspension (FISH-IS) assay for the ImageStream. Dr. Maguire has active membership in the American Association for Cancer Research (AACR) and the International Society for Advancement of Cytometry (ISAC).

  Dr. Brian Eric Hall Title / Designation: Senior Scientist Institution: Merck Topic of the presentation: Protein Aggregation and Applications using the ImageStream High Content Analyzer Seminar Abstract: The ImageStream is a multispectral imaging flow cytometer that generates high resolution images of cells at a rate of 1000's of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. With this novel approach the ImageStream is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring the apoptotic index and imaging of rare cell populations. New assay development also allows for the enumeration of protein aggregates and silicon oil droplets, a critical quality control assay for protein based pharmaceuticals and reagents. Speaker Biography: Dr.Brian Hall is a senior scientist at Amnis Corporation and has been working on the ImageStream technology for the past 15 years. As one of the original Amnis team members, he's gained the experience of developing the hardware systems from a prototype, helped write the technical manuals, designed the training courses and conduced seminars and poster presentations the world over. He has 4 patents and over 18 publications using quantitative image Cytometry and continues to collaborate with researchers developing novel applications for the ImageStream. He is a graduate of Boston University, and spent the early part of his career doing cancer research at both the Fox Chase Cancer Research Center in Philadelphia and the Fred Hutchinson Cancer Center in Seattle. After specializing in Flow Cytometry and confocal microscopy he moved to Amnis.

  周家喜(Jiaxi Zhou) Institution & Title: 协和医科大学特聘教授 协和医科大学天津血液病研究所科技处副处长(Prof, Peking Union Medical College) Topic of the presentation: "造血细胞早期分化机理研究"(The study on the early differentiation mechanism of hematopoietic cell)

  杨明(Ming Yang) Institution & Title: 北京工业大学副教授 北京市科技新星(Associate Prof, Beijing University of Technology) Topic of the presentation: "抗肿瘤药物筛选"(Anti-tumor drug screening)

• Agenda

  
  

  TIME	TOPICS	SPEAKER(S)
  9:00 - 9:30am	Registration & Seating
  9:30 - 9:50am	Welcome & Introductions
  9:50 - 10:50am	Academic Presentation "Addressing intracellular routing of antigens in antigen-presenting cells using imaging flow cytometry"	Juan Garcia Vallejo, MD, Ph.D Senior Researcher, Department of Molecular Cell Biology & Immunology VU University Medical Center, Netherlands
  10:50 - 11:10am	Tea Break & Poster Session
  11:10 - 12:10pm	Academic Presentation "Clinical applications of imaging flow cytometry"	Orla Maguire, Ph.D Research Scientist Roswell Park Cancer Institute, USA
  12:10 - 13:00pm	Lunch
  13:00 - 13:30pm	Registration & Seating
  13:30 - 14:30pm	Academic Presentation "Protein Aggregation and Applications using the ImageStream High Content Analyzer"	Dr. Brian Eric Hall Senior Scientist Merck, USA
  14:30 - 14:50pm	Tea Break & Poster Session
  14:50 - 15:50pm	Academic Presentation "造血细胞早期分化机理研究"(The study on the early differentiation mechanism of hematopoietic cell)	周家喜(Jiaxi Zhou) 协和医科大学特聘教授 协和医科大学天津血液病研究所科技处副处长 （Prof, Peking Union Medical College ）
  15:50 - 16:50pm	Academic Presentation "抗肿瘤药物筛选"(Anti-tumor drug screening)	杨明(Ming Yang) 北京工业大学副教授 北京市科技新星 (Associate Prof, Beijing University of Technology)
  16:50 - 17:00pm	Ending & Acknowledge
  17:00 - 17:20pm	Dry Demo Show

April 16, Shanghai

• Speaker list and biography

  Juan Garcia Vallejo, MD, Ph.D. Title / Designation: Senior Researcher, Department of Molecular Cell Biology & Immunology Institution: VU University Medical Center Topic of the presentation: Addressing intracellular routing of antigens in antigen-presenting cells using imaging flow cytometry Speaker Biography: Dr. Garcia-Vallejo obtained his MD from the University of Malaga (Spain) in 1999 and quickly moved into basic research in the field of Glycobiology and Immunology. His PhD dissertation on the regulation of glycosylation in endothelial cells was defended in 2005 at the Free University of Amsterdam. Dr. Garcia-Vallejo moved then to the group of Prof. van Kooyk at the VU University Medical Center, where he has worked since in the Immunobiology of C-type lectin mediated antigen uptake in dendritic cells, with a special interest in their role in the control of immune homeostasis and their potential in cancer immunotherapy.

  Orla Maguire, Ph.D. Title / Designation: Research Scientist （Flow Cytometry Specialist） Institution: Roswell Park Cancer Institute Topic of the presentation: Clinical applications of imaging flow cytometry Speaker Biography: Dr. Orla Maguire obtained her B.Sc. and Ph.D in Biomedical Science in 2004 and 2008, respectively from the University Of Ulster, Northern Ireland, UK. She started her post-Doc work in Roswell Park Cancer Institute, Buffalo, NY, USA initially with focus on Pharmacology, and then Imaging Flow Cytometry and she is now a Flow Cytometry Specialist there. Dr. Maguire is particularly interested in the clinical applications of imaging flow cytometry and has published her research on quantification of the NF-κB and NFAT signaling pathway using the ImageStream. She has also developed a novel Fluorescent In Situ Hybridization In Suspension (FISH-IS) assay for the ImageStream. Dr. Maguire has active membership in the American Association for Cancer Research (AACR) and the International Society for Advancement of Cytometry (ISAC).

  Dr. Brian Eric Hall Title / Designation: Senior Scientist Institution: Merck Topic of the presentation: Protein Aggregation and Applications using the ImageStream High Content Analyzer Seminar Abstract: The ImageStream is a multispectral imaging flow cytometer that generates high resolution images of cells at a rate of 1000's of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. With this novel approach the ImageStream is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring the apoptotic index and imaging of rare cell populations. New assay development also allows for the enumeration of protein aggregates and silicon oil droplets, a critical quality control assay for protein based pharmaceuticals and reagents. Speaker Biography: Dr.Brian Hall is a senior scientist at Amnis Corporation and has been working on the ImageStream technology for the past 15 years. As one of the original Amnis team members, he's gained the experience of developing the hardware systems from a prototype, helped write the technical manuals, designed the training courses and conduced seminars and poster presentations the world over. He has 4 patents and over 18 publications using quantitative image Cytometry and continues to collaborate with researchers developing novel applications for the ImageStream. He is a graduate of Boston University, and spent the early part of his career doing cancer research at both the Fox Chase Cancer Research Center in Philadelphia and the Fred Hutchinson Cancer Center in Seattle. After specializing in Flow Cytometry and confocal microscopy he moved to Amnis.

  王宾(Bin Wang) Institution & Title: 复旦大学医学院教授(Prof, Medical School, Fudan University) Topic of the presentation: "成像型流式在自身免疫疫病研究中的应用"(Imaging Flow Application in the Auto-immune Disease Research)

• Agenda

  
  

  TIME	TOPICS	SPEAKER(S)
  9:00 - 9:30am	Registration & Seating
  9:30 - 9:50am	Welcome & Introductions
  9:50 - 10:50am	Academic Presentation "Addressing intracellular routing of antigens in antigen-presenting cells using imaging flow cytometry"	Juan Garcia Vallejo, MD, Ph.D Senior Researcher, Department of Molecular Cell Biology & Immunology VU University Medical Center, Netherlands
  10:50 - 11:10am	Tea Break & Poster Session
  11:10 - 12:10pm	Academic Presentation "Clinical applications of imaging flow cytometry"	Orla Maguire, Ph.D Research Scientist Roswell Park Cancer Institute, USA
  12:10 - 13:00pm	Lunch
  13:00 - 13:30pm	Registration & Seating
  13:30 - 14:30pm	Academic Presentation "Protein Aggregation and Applications using the ImageStream High Content Analyzer"	Dr. Brian Eric Hall Senior Scientist Merck, USA
  14:30 - 14:50pm	Tea Break & Poster Session
  14:50 - 15:50pm	Academic Presentation "成像型流式在自身免疫疫病研究中的应用"(Imaging Flow Application in the Auto-immune Disease Research)	王宾教授(Bin Wang, Prof) 复旦大学医学院(Medical School, Fudan University)
  15:50 - 16:00pm	Ending & Acknowledge
  16:00 - 16:20pm	Dry Demo Show

April 20, Tokyo

• Speaker list and biography

  Juan Garcia Vallejo, MD, Ph.D. Title / Designation: Senior Researcher, Department of Molecular Cell Biology & Immunology Institution: VU University Medical Center Topic of the presentation: Addressing intracellular routing of antigens in antigen-presenting cells using imaging flow cytometry Speaker Biography: Dr. Garcia-Vallejo obtained his MD from the University of Malaga (Spain) in 1999 and quickly moved into basic research in the field of Glycobiology and Immunology. His PhD dissertation on the regulation of glycosylation in endothelial cells was defended in 2005 at the Free University of Amsterdam. Dr. Garcia-Vallejo moved then to the group of Prof. van Kooyk at the VU University Medical Center, where he has worked since in the Immunobiology of C-type lectin mediated antigen uptake in dendritic cells, with a special interest in their role in the control of immune homeostasis and their potential in cancer immunotherapy.

  Orla Maguire, Ph.D. Title / Designation: Research Scientist （Flow Cytometry Specialist） Institution: Roswell Park Cancer Institute Topic of the presentation: Clinical applications of imaging flow cytometry Speaker Biography: Dr. Orla Maguire obtained her B.Sc. and Ph.D in Biomedical Science in 2004 and 2008, respectively from the University Of Ulster, Northern Ireland, UK. She started her post-Doc work in Roswell Park Cancer Institute, Buffalo, NY, USA initially with focus on Pharmacology, and then Imaging Flow Cytometry and she is now a Flow Cytometry Specialist there. Dr. Maguire is particularly interested in the clinical applications of imaging flow cytometry and has published her research on quantification of the NF-κB and NFAT signaling pathway using the ImageStream. She has also developed a novel Fluorescent In Situ Hybridization In Suspension (FISH-IS) assay for the ImageStream. Dr. Maguire has active membership in the American Association for Cancer Research (AACR) and the International Society for Advancement of Cytometry (ISAC).

  Dr. Brian Eric Hall Title / Designation: Senior Scientist Institution: Merck Topic of the presentation: Protein Aggregation and Applications using the ImageStream High Content Analyzer Seminar Abstract: The ImageStream is a multispectral imaging flow cytometer that generates high resolution images of cells at a rate of 1000's of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. With this novel approach the ImageStream is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring the apoptotic index and imaging of rare cell populations. New assay development also allows for the enumeration of protein aggregates and silicon oil droplets, a critical quality control assay for protein based pharmaceuticals and reagents. Speaker Biography: Dr.Brian Hall is a senior scientist at Amnis Corporation and has been working on the ImageStream technology for the past 15 years. As one of the original Amnis team members, he's gained the experience of developing the hardware systems from a prototype, helped write the technical manuals, designed the training courses and conduced seminars and poster presentations the world over. He has 4 patents and over 18 publications using quantitative image Cytometry and continues to collaborate with researchers developing novel applications for the ImageStream. He is a graduate of Boston University, and spent the early part of his career doing cancer research at both the Fox Chase Cancer Research Center in Philadelphia and the Fred Hutchinson Cancer Center in Seattle. After specializing in Flow Cytometry and confocal microscopy he moved to Amnis.

• Agenda