| Method | ISO 10272-1 | FDA BAM Chapter 7 | FSIS MLG 41.00 |
|---|---|---|---|
| Pre-enrichment | Bolton Broth, 37 ± 1°C; 4-6 h, microaerophilic |
Bolton Broth, 37 ± 1°C; 4 h, Microaerophilic or 30°C, 3 h + 37°C, 2 h, microaerophilic |
N/A |
| Selective enrichment | Bolton Broth 41.5°C, 44 ± 4 h microaerophilic |
Bolton Broth 42°C, 20 and 44 ± 4 h microaerophilic |
BPW + 2 x Blood-Free Bolton Broth, 42 ± 1°C; 48 ± 2 h, microaerophilic |
| Isolation | mCCD agar + additional, 41.5°C; 44 ± 4 h, microaerophilic |
mCCD agar or AHB Agar, 37 - 42°C; 24 - 48 h, microaerophilic |
Campy-Cefex Agar, 42 ± 1°C; 48 ± 2 h, microaerophilic |
Rapid Testing Solutions for the
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Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide. Infections are usually caused by consuming cross-contaminated or insufficiently processed food (typically red meat, poultry, shellfish and unpasteurized milk). There is an increasing demand for Campylobacter testing in food to meet new regulations regarding contamination levels in poultry.
A comprehensive selection of products is available from Merck for the enrichment, detection and isolation of Campylobacter spp. These include:
- Dehydrated Culture Media: Unique and convenient granulated media meeting the highest industry performance standards as laid out in ISO 11133 and
- Singlepath® Lateral Flow Tests: Innovative immunoassays in the format of “pregnancy tests” for detection with ultimate convenience in as little as 20 minutes after enrichment.
The do-it-yourself kit for on-site immunological screening of Campylobacter in poultry flocks requires no prior enrichment step and delivers results within only 2 hours. For increased reliability, Singlepath® Direct Campy Poultry Kit includes a built-in control reaction. There is no need to outsource testing and wait several days for the results or to invest in capital-intensive instrumentation.
