70563 Sigma-AldrichpET-42c(+) DNA
Empfohlene Produkte
Übersicht
| Replacement Information |
|---|
| Description | |
|---|---|
| Overview | This product has been discontinued. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow removal of 100% of the vector-encoded sequences and the generation of native proteins with their authentic N-terminal residues. Unfused proteins can be produced by using the Nde I cloning site. A version of pET-41 is available as a linearized vector prepared for ligation-independent cloning (LIC) of PCR products. Another pET vector with the GST•Tag sequence is pET-49b(+). Please see the website description for more information. The His•Tag and S•Tag sequences enable alternative protein detection and purification procedures to be performed. For example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.  The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB239). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase. The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product. |
| Catalogue Number | 70563 |
| Brand Family | Novagen® |
| References | |
|---|---|
| References | Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31. |
| Product Information |
|---|
| Applications |
|---|
| Biological Information |
|---|
| Physicochemical Information |
|---|
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information |
|---|
| Product Usage Statements |
|---|
| Storage and Shipping Information | |
|---|---|
| Ship Code | Shipped with Blue Ice or with Dry Ice |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Packaging Information |
|---|
| Transport Information |
|---|
| Supplemental Information |
|---|
| Specifications |
|---|
| Global Trade Item Number | |
|---|---|
| Bestellnummer | GTIN |
| 70563 | 0 |
Documentation
pET-42c(+) DNA Analysenzertifikate
| Titel | Chargennummer |
|---|---|
| 70563 |
Literatur
| Übersicht |
|---|
Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31. |
Broschüre
| Titel |
|---|
| The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression |
Anwendungsvorschriften
| Titel |
|---|
| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB055 pET System Manual |
Vektor-Karte
| Titel |
|---|
| TB240VM pET-42a-c(+) Vector Map |
Vektorsequenz
| Titel |
|---|
| pET-42c(+) Sequence |