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475987 mTOR, Human, Recombinant, S. frugiperda

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      Übersicht

      Replacement Information
      Description
      OverviewRecombinant, human mTOR consisting of amino acids 1360-2549 fused at the N-terminus to DYKDDDDK sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. mTOR is a ubiquitously expressed, serine/threonine protein kinase regulated by growth factor signaling, amino acid availability, and energy levels. mTOR activity is inhibited by rapamycin as well as broad spectrum PI 3-kinase inhibitors. The catalytically active enzyme is useful for inhibitor screening. Active in kinase assay using the K-LISA™ mTOR (Recombinant) Activity Kit, Cat. No. CBA104.

      This product has been discontinued.
      Catalogue Number475987
      Brand Family Calbiochem®
      Application Data
      Increasing amounts of mTOR, Human, Recombinant, S. frugiperda were assayed for mTOR activity using the K-LISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

      The activity of mTOR, Human, Recombinant, S. frugiperda was assaying in the presence of increasing amounts of Wortmannin (Cat. No. 681675) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

      The activity of mTOR, Human, Recombinant, S. frugiperda was assayed in the presence of increasing concentrations of Rapamycin (Cat. No. 553210) and in the presence (+) or absence (-) of FKBP12, GST-Fusion, Human, Recombinant, E. coli (325902) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.
      References
      ReferencesSarbassov, D.D., et al. 2005. Science 307, 1098.
      Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
      Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
      Kim, D.H., et al. 2002. Cell 110,163.
      Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
      Brown, E.J., et al. 1994. Nature 369,756.
      Sabatini, D.M., et al. 1994. Cell 78, 35.
      Heitman, J., et al. 1991. Science 253, 905.
      Product Information
      FormLiquid
      FormulationIn 50 mM NaCl, 50 mM β-glycerophosphate, 10 mM HEPES, 500 µM TCEP, 10% glycerol, 0.03% Brij-®35 detergent, 200 µg/ml DYKDDDDK Peptide, pH 7.4.
      Applications
      Biological Information
      Purity≥50% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      475987 0

      Documentation

      mTOR, Human, Recombinant, S. frugiperda Analysenzertifikate

      TitelChargennummer
      475987

      Literatur

      Übersicht
      Sarbassov, D.D., et al. 2005. Science 307, 1098.
      Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
      Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
      Kim, D.H., et al. 2002. Cell 110,163.
      Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
      Brown, E.J., et al. 1994. Nature 369,756.
      Sabatini, D.M., et al. 1994. Cell 78, 35.
      Heitman, J., et al. 1991. Science 253, 905.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-April-2009 JSW
      Application Data
      Increasing amounts of mTOR, Human, Recombinant, S. frugiperda were assayed for mTOR activity using the K-LISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

      The activity of mTOR, Human, Recombinant, S. frugiperda was assaying in the presence of increasing amounts of Wortmannin (Cat. No. 681675) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

      The activity of mTOR, Human, Recombinant, S. frugiperda was assayed in the presence of increasing concentrations of Rapamycin (Cat. No. 553210) and in the presence (+) or absence (-) of FKBP12, GST-Fusion, Human, Recombinant, E. coli (325902) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.
      DescriptionRecombinant, human mTOR consisting of amino acids 1360-2549 fused at the N-terminus to DYKDDDDK sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. mTOR is a ubiquitously expressed, serine/threonine protein kinase regulated by growth factor signaling, amino acid availability, and energy levels. mTOR activity is inhibited by rapamycin as well as broad spectrum PI 3-kinase inhibitors. The catalytically active enzyme is useful for inhibitor screening. Active in kinase assay using the K-LISA™ mTOR (Recombinant) Activity Kit, Cat. No. CBA104.
      FormLiquid
      FormulationIn 50 mM NaCl, 50 mM β-glycerophosphate, 10 mM HEPES, 500 µM TCEP, 10% glycerol, 0.03% Brij-®35 detergent, 200 µg/ml DYKDDDDK Peptide, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥50% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesSarbassov, D.D., et al. 2005. Science 307, 1098.
      Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
      Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
      Kim, D.H., et al. 2002. Cell 110,163.
      Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
      Brown, E.J., et al. 1994. Nature 369,756.
      Sabatini, D.M., et al. 1994. Cell 78, 35.
      Heitman, J., et al. 1991. Science 253, 905.